Keratin 5/8 antibody

Catalog No. ABIN6174487

Product Details anti-Keratin 5/8 Antibody

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Purpose: Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), Purified, BSA-free

Characteristics: This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Cytoskeletal preparation from HeLa cells

Clone: C-50

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Western blotting 0.5-1.0 μg/mL
• Does not react with chicken , or Xenopus
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2 or A431 cells. Skin or Squamous cell carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: Cytokeratin 5/8 (KRT5/8 Products)

Molecular Weight: 58 kDa (CK5), 52.5 kDa (CK8)

Gene ID: 3852, 3856