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CKB antibody
CKB
Reactivity: Human
WB, IF, IHC (fro), FACS
Host: Mouse
Monoclonal
2ba6
unconjugated
Product Details anti-CKB Antibody
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Target
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CKB
(Creatine Kinase, Brain (CKB))
Reactivity
All reactivities for CKB antibodies
Human
Host
All hosts for CKB antibodies
Mouse
Clonality
All clonalities for CKB antibodies
Monoclonal
Conjugate
All conjugates for CKB antibodies
This CKB antibody is un-conjugated
Application
All applications for CKB antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
Purification
Protein G affinity chromatography
Immunogen
Human CKBB protein was used as the immunogen for the Creatine kinase B type antibody.
Clone
2ba6
Isotype
IgG1 kappa
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Alternatives
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Application Details
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Application Notes
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml Immunofluorescence: 0.5-1 μg/mL Immunohistochemistry (Frozen Only): 0.5-1 μg/mL for 30 min at RT Optimal dilution of the Creatine kinase B type antibody should be determined by the researcher.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Target Details for CKB
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Target
CKB
(Creatine Kinase, Brain (CKB))
Alternative Name
Creatine kinase B type (CKB Products )
Synonyms
B-CK antibody, CKBB antibody, b-ck antibody, ckbb antibody, CKB antibody, Bck antibody, Ck-3 antibody, Ck3 antibody, Ckbb antibody, bck antibody, ckb antibody, cb594 antibody, wu:fj36f10 antibody, wu:fj37d11 antibody, Ckbr antibody, mg:cb02h12 antibody, wu:fb62d04 antibody, wu:fc14g11 antibody, zgc:154095 antibody, zgc:92079 antibody, creatine kinase B antibody, creatine kinase, brain L homeolog antibody, creatine kinase B-type antibody, creatine kinase, brain antibody, creatine kinase, brain b antibody, creatine kinase, brain a antibody, CKB antibody, ckb.L antibody, Ckb antibody, ckb antibody, ckbb antibody, ckba antibody
Background
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This mAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins
Gene ID
1152
UniProt
P12277
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