CDKN1B antibody
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- Target See all CDKN1B Antibodies
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
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Reactivity
- Human, Mouse, Rat, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CDKN1B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purification
- Protein G affinity chromatography
- Immunogen
- Purified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
- Clone
- SX53G8
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CDKN1B Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
- Alternative Name
- p27Kip1 (CDKN1B Products)
- Synonyms
- CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody
- Background
- This antibody recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. The p27Kip1 antibody is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. It is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
- Gene ID
- 1027
- UniProt
- P46527
- Pathways
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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