Keratin 5/8 antibody

Catalog No. ABIN6252350

Product Details anti-Keratin 5/8 Antibody

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Does not react with Chicken & Xenopus.

Purification: Protein G affinity chromatography

Immunogen: A cytoskeletal preparation from HeLa cells was used as the immunogen for the Cytokeratin 5/8 antibody.

Clone: SPM268

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the Cytokeratin 5/8 antibody should be determined by the researcher.

1. For staining of formalin-fixed tissues, digest sections with Trypsin at 1 mg/mL PBS, 15 min at RT
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: Cytokeratin 5/8 (KRT5/8 Products)

Background: It reacts with keratin 5 (58  kDa) and keratin 8 (52.5  kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Gene ID: 3852, 3856

UniProt: P13647, P05787