BAX antibody (AA 3-16)
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- Target See all BAX Antibodies
- BAX (BCL2-Associated X Protein (BAX))
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Binding Specificity
- AA 3-16
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Reactivity
- Human, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This BAX antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Does not react with mouse and rat
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.
- Clone
- SPM336
- Isotype
- IgG1
- Top Product
- Discover our top product BAX Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- BAX (BCL2-Associated X Protein (BAX))
- Alternative Name
- Bax (BAX Products)
- Synonyms
- BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody
- Background
- Recognizes a protein of 21 kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
- Gene ID
- 581
- UniProt
- Q07812
- Pathways
- p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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