TRPM8 antibody

Catalog No. ABIN6265758

Product Details anti-TRPM8 Antibody

Target: TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPM8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: TRPM8 Antibody detects endogenous levels of total TRPM8

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TRPM8

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for TRPM8

Target: TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))

Alternative Name: TRPM8 (TRPM8 Products)

Synonyms: TRPM8 antibody, trpp8 antibody, ltrpc6 antibody, trpm8b antibody, LTRPC6 antibody, TRPP8 antibody, CMR1 antibody, LTrpC-6 antibody, Trp-p8 antibody, transient receptor potential cation channel subfamily M member 8 antibody, transient receptor potential cation channel subfamily M member 8b L homeolog antibody, transient receptor potential cation channel, subfamily M, member 8 antibody, TRPM8 antibody, trpm8b.L antibody, Trpm8 antibody

Background:

Description: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular  pH . Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186). Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel.

Gene: TRPM8

Molecular Weight: 128 kDa

Gene ID: 79054

UniProt: Q7Z2W7