Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Histone 3 antibody (H3K9me2)

H3 Reactivity: Human, Mouse WB, IHC, IF, ELISA, ChIP, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6266177
  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 59
    • 47
    • 45
    • 42
    • 41
    • 36
    • 36
    • 35
    • 35
    • 35
    • 34
    • 32
    • 31
    • 29
    • 29
    • 29
    • 26
    • 25
    • 23
    • 23
    • 23
    • 22
    • 20
    • 18
    • 18
    • 17
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 12
    • 12
    • 12
    • 12
    • 11
    • 11
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    H3K9me2
    Reactivity
    • 1399
    • 940
    • 861
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 30
    • 29
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 1217
    • 243
    • 8
    • 4
    Rabbit
    Clonality
    • 1120
    • 351
    • 1
    Polyclonal
    Conjugate
    • 821
    • 81
    • 63
    • 63
    • 63
    • 62
    • 62
    • 62
    • 37
    • 33
    • 23
    • 17
    • 17
    • 17
    • 17
    • 17
    • 14
    • 2
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1058
    • 410
    • 378
    • 312
    • 251
    • 188
    • 181
    • 178
    • 163
    • 161
    • 141
    • 127
    • 119
    • 48
    • 38
    • 34
    • 32
    • 20
    • 16
    • 14
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificity
    Di-Methyl-Histone H3 (Lys9)/H3K9me2 Antibody detects endogenous levels of Di-Methyl-Histone H3 only when methylated at Lys9.
    Predicted Reactivity
    Horse,Sheep,Rabbit
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthetic methylated peptide derived from human Di-Methyl-Histone H3 around the methylation site of Lys9.
    Isotype
    IgG
    Top Product
    Discover our top product H3 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Wu, Huang, Jiao, Ding, Zhang, Chen, Wang, Li, Huo: "Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells." in: Oncotarget, Vol. 8, Issue 51, pp. 88630-88644, (2017) (PubMed).

  • Target
    Histone 3 (H3)
    Alternative Name
    HIST3H3 (H3 Products)
    Synonyms
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    Background

    Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    Gene: HIST3H3

    Molecular Weight
    15kDa
    Gene ID
    8290
    UniProt
    Q16695
You are here:
Support