PERK antibody (AA 920-1000)

Catalog No. ABIN6285398

Product Details anti-PERK Antibody

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Binding Specificity: AA 920-1000

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PERK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PERK is a protein encoded by the EIF2AK3 gene which is approximately 125,2 kDa. PERK is localised to the endoplasmic reticulum membrane. It is involved in respiratory electron transport, apoptosis modulation and signalling and animal autophagy. It phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and a rapid reduction of translational initiation and repression of global protein synthesis. PERK is expressed in the nervous system, liver, pancreas, lung and heart. Mutations in the EIF2AK3 gene may result in Wolcott-Rallison syndrome and diversion colitis. STJ95031 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PERK protein only when phosphorylated at T981.

Specificity: PERK Polyclonal Antibody detects endogenous levels of PERK protein.

Characteristics: Rabbit polyclonal to PERK.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PERK around the non-phosphorylation site of T981.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Comment:

Ubiquitous. A high level expression is seen in secretory tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PERK

Target: PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

Alternative Name: PERK (EIF2AK3 Products)

Synonyms: EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody

Molecular Weight: 125 kDa

Gene ID: 9451

UniProt: Q9NZJ5

Pathways: Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response