c-MYC antibody

Catalog No. ABIN6296156

The Mouse Monoclonal anti-c-MYC antibody is suitable to detect c-MYC in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for c-MYC antibody (ABIN6296156)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-c-MYC Antibody

Purpose: Mouse anti-Human/Mouse/Rat c-Myc Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA were used as the immunogen for the c-Myc antibody.

Immunogen: Amino acids AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA were used as the immunogen for the c-Myc antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: Myc proto-oncogene protein

Background:

Target Description: It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This mAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Gene Symbol: MYC///BHLHE39

Gene ID: 4609

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect