Vimentin antibody (CF®488A)
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human, Pig, Cow, Chicken, Dog, Cat, Goat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is conjugated to CF®488A
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)
- Specificity
- This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human vimentin protein
- Clone
- VM452
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Positive Control: Jurkat cells, Sarcomas, Melanomas.
Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 100 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA and 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- Vimentin (VIM)
- Alternative Name
- VIM (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Molecular Weight
- 57-60kDa
- Gene ID
- 7431
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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