Vimentin antibody
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Human. Cow. Dog. Cat. Pig. Goat. Chicken. Does not react with Mouse or Rat.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human vimentin protein
- Clone
- VM452
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product VIM Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Vimentin (VIM)
- Alternative Name
- VIM (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Background
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VIM,Vimentin (Mesenchymal Cell Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 57-60kDa
- Gene ID
- 7431, 455493
- UniProt
- P08670
- Pathways
- Caspase Cascade in Apoptosis
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