PCNA antibody
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- Target See all PCNA Antibodies
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PCNA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
- Cross-Reactivity (Details)
- Shows broad species reactivity.
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant full length human PCNA protein
- Clone
- PCNA-694
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product PCNA Primary Antibody
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- Application Notes
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Positive Control: Tonsil or reactive lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
- Alternative Name
- PCNA (PCNA Products)
- Synonyms
- PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody
- Molecular Weight
- 36kDa
- Gene ID
- 5111
- UniProt
- P12004
- Pathways
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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