ERK1 antibody

Catalog No. ABIN6943273

Product Details anti-ERK1 Antibody

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: Recombinant human ERK1 around N terminus

Clone: 6B4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1 (MAPK3 Products)

Synonyms: ERK-1 antibody, ERK1 antibody, ERT2 antibody, HS44KDAP antibody, HUMKER1A antibody, P44ERK1 antibody, P44MAPK antibody, PRKM3 antibody, p44-ERK1 antibody, p44-MAPK antibody, Erk-1 antibody, Erk1 antibody, Ert2 antibody, Esrk1 antibody, Mnk1 antibody, Mtap2k antibody, Prkm3 antibody, p44 antibody, p44erk1 antibody, p44mapk antibody, ERK3 antibody, ERK6 antibody, P38GAMMA antibody, PRKM12 antibody, SAPK-3 antibody, SAPK3 antibody, fi06b09 antibody, wu:fi06b09 antibody, zERK1 antibody, Tb08.10J17.940 antibody, MAPK1 antibody, MNK1 antibody, AW123708 antibody, Erk6 antibody, P38gamma antibody, Prkm12 antibody, Sapk3 antibody, ATMAPK3 antibody, ATMPK3 antibody, T6D9.4 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 12 antibody, mitogen activated protein kinase 3 antibody, mitogen-activated serine/threonine-protein kinase antibody, MAPK3 antibody, Mapk3 antibody, MAPK12 antibody, mapk3 antibody, Tc00.1047053509475.10 antibody, Tb927.8.3550 antibody, Mapk12 antibody, CEK1 antibody, MPK3 antibody

Background:

Synonyms: Mitogen-activated protein kinase 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, MAP kinase 3, MAPK 3, ERK-1, p44-MAPK, MAPK3

Background: p44/42 MAP Kinase(Thr202), ERK (extracellular signal regulated kinase), also known as MAPK (mitogen activated protein kinase) has two closely related isoforms of 44 kDa and 42 kDa, respectively. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with a large variety of stimuli including mitogens, hormones, growth factors, cytokines, and bioactive peptides. Cell stimulation induces the activation of a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as the cytoskeleton. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating the activity of ERK1&2.

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins