Histone 3 antibody (acLys14)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- AA 10-18, acLys14
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq)
- Characteristics
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization. Histone H3K14ac antibody (mAb) (Clone 13HH3-1A5) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
- Purification
- Protein A Chromatography
- Immunogen
- This antibody was raised against a peptide corresponding to amino acids 10-18 including acetyl-lysine 14 of human Histone H3.
- Clone
- 13HH3-1A5
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in 60 mM Sodium Citrate, 105 mM Tris-HCl, 30 % glycerol, and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Molecular Weight
- 17 kDa
- NCBI Accession
- NP_003522
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