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Histone 3 antibody (2meArg8 (asymetric))

H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6972053
  • Target See all Histone 3 (H3) Antibodies
    Histone 3 (H3)
    Binding Specificity
    • 56
    • 47
    • 44
    • 41
    • 40
    • 36
    • 35
    • 35
    • 34
    • 34
    • 34
    • 32
    • 29
    • 29
    • 28
    • 27
    • 26
    • 25
    • 23
    • 22
    • 22
    • 22
    • 20
    • 18
    • 18
    • 17
    • 16
    • 16
    • 16
    • 16
    • 16
    • 16
    • 15
    • 14
    • 14
    • 13
    • 13
    • 12
    • 12
    • 12
    • 11
    • 11
    • 11
    • 10
    • 10
    • 10
    • 10
    • 9
    • 9
    • 8
    2meArg8 (asymetric)
    Reactivity
    • 1368
    • 938
    • 860
    • 59
    • 42
    • 41
    • 41
    • 40
    • 39
    • 35
    • 35
    • 29
    • 26
    • 22
    • 19
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 1183
    • 242
    • 8
    • 4
    Rabbit
    Clonality
    • 1108
    • 328
    • 1
    Polyclonal
    Conjugate
    • 795
    • 81
    • 63
    • 63
    • 63
    • 62
    • 62
    • 62
    • 34
    • 33
    • 21
    • 17
    • 17
    • 17
    • 17
    • 17
    • 12
    • 1
    This Histone 3 antibody is un-conjugated
    Application
    • 1034
    • 402
    • 373
    • 311
    • 239
    • 188
    • 175
    • 166
    • 163
    • 150
    • 137
    • 126
    • 117
    • 48
    • 38
    • 34
    • 32
    • 20
    • 16
    • 14
    • 7
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)
    Characteristics
    Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation. Histone H3R8me2a (asymmetric) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Dot blot and Western blot, it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
    Purification
    Affinity Purified
    Immunogen
    This Histone H3 dimethyl Arg8 asymmetric antibody was raised against a peptide containing dimethyl-Arg8 (asymmetric) of human histone H3.
    Isotype
    IgG
    Top Product
    Discover our top product H3 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • Target
    Histone 3 (H3)
    Alternative Name
    Histone H3 (H3 Products)
    Synonyms
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    Molecular Weight
    17 kDa
    NCBI Accession
    NP_003522
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