Histone 3 antibody (pThr45)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- pThr45
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Reactivity
- Human, Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Dot Blot (DB)
- Characteristics
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of histone H2A, histone H2B, histone H3 and histone H4). Histones are subject to a number of post-translational modifications, including acetylation, phosphorylation and methylation. In budding yeast, H3 Thr45 phosphorylation occurs specifically during DNA replication, and is catalyzed by the Cdc7-Dbf4 kinase. Yeast that lack threonine 45 exhibit defects in replication, suggesting that phosphorylation of Thr45 is associated with DNA replication. Threonine 45 lies within a conserved region of histone H3 that makes critical contacts with DNA when assembled into chromatin. It is located at the points of entry and exit of DNA on the nucleosome, and phosphorylation may influence histone-DNA interactions within the nucleosome. Histone H3T45ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot, it has been shown to react with Budding Yeast and Human samples, but it is predicted that it will react with a wide range of sample types.
- Purification
- Affinity Purified
- Immunogen
- This Histone H3 phospho Thr45 antibody was raised against a peptide containing phospho Thr45 of human histone H3.
- Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- Molecular Weight
- 17 kDa
- NCBI Accession
- NP_003522
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