CHEK1 antibody (pSer286) (Alexa Fluor 594)

Catalog No. ABIN6979062

Product Details anti-CHEK1 Antibody (Alexa Fluor 594)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Binding Specificity: pSer286

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is conjugated to Alexa Fluor 594

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Dog,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser286.

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1 (CHEK1 Products)

Synonyms: An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

Background:

Synonyms: Chk1 (phospho S286), p-Chk1 (phospho S286), Chk1 (Phospho-Ser286), CHEK1 (phospho Ser286), CHEK1 (phospho Ser286), p-CHEK1 (Ser286), Cell cycle checkpoint kinase, Checkpoint, S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, CHEK-1, CHEK1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae), C85740, CHK1_HUMAN.

Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2011].

Gene ID: 1111

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair