TANK Antibody is affinity chromatography purified via peptide column.
Immunogen
TANK antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TANK. The immunogen is located within amino acids 350 - 400 of TANK.
TANK
Reactivity: Human
WB, IF, EIA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
TANK antibody can be used for the detection of TANK by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
TANK Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
TANK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
TANK
(TRAF Family Member-Associated NFKB Activator (TANK))
I-TRAF antibody, ITRAF antibody, TRAF2 antibody, C86182 antibody, E430026L09Rik antibody, zgc:153048 antibody, i-traf antibody, tank antibody, traf2 antibody, TRAF family member associated NFKB activator antibody, TRAF family member-associated Nf-kappa B activator antibody, TRAF family member-associated NFKB activator antibody, TRAF family member associated NFKB activator L homeolog antibody, TANK antibody, Tank antibody, tank antibody, tank.L antibody
Background
TANK Antibody: TANK was initially identified as a novel TRAF-interacting protein that regulated TRAF-mediated signal transduction. Specifically, ligand binding by surface receptors in the tumor necrosis factor (TNF) receptor and Toll/interleukin-1 (IL-1) receptor families lead to the formation of a TRAF/TANK complex that mediates the activation of the transcription factor NF-κ,B. This activation of NF-κ,B occurs through an association with the kinases IKKε, and TBK1. More recently, it was shown that these proteins can then form a complex with NEMO, a protein that regulates the activity of the Iκ,B complex. This suggests that in addition to the possibility that TBK1 and IKKε, activate the IKKs, the association with the IKK complex may help these kinases modulate other functions, such as the transactivation potential of NF-κ,B proteins. At least two isoforms of TANK are known to exist.