ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
NOD4 antibody is predicted to not cross-react with other members of the NOD family of proteins.
Purification
NOD4 Antibody is affinity chromatography purified via peptide column.
Immunogen
NOD4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NOD4. The immunogen is located within amino acids 20 - 70 of NOD4.
NLRC5
Reactivity: Human
ELISA, WB, IF, IC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
NOD4 antibody can be used for detection of NOD4 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NOD4 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NOD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NOD4 Antibody: NOD4 is a member of the NOD (nucleotide-binding oligomerization domain) family, a group of proteins that are involved in innate immune defense. NOD4 contains a CARD-like domain, a central NOD domain and a large LRR region. NOD4, an IFN-gamma-inducible nuclear protein, plays a role in homeostatic control of innate immunity and in antiviral defense mechanisms. As a key negative regulator of NF-κ,B and type I interferon signaling, NOD4 may be a useful target for manipulating immune responses against infectious or inflammation-associated diseases, including cancer.