ELISA, Western Blotting (WB), Immunofluorescence (IF)
Purification
NALP6 Antibody is affinity chromatography purified via peptide column.
Immunogen
NALP6 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NALP6. The immunogen is located within amino acids 150 - 200 of NALP6.
NLRP6
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
NALP6 antibody can be used for detection of NALP6 by Western blot at 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
NALP6 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
NALP6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
NALP6 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP6, also known as PYPAF5, is highly expressed in granulocytes and T cells and co-localizes with the PYRIN-CARD protein ASC. Co-expression of NALP6 with ASC results in a synergistic activation of NF-κ,B and activated caspase-1-dependent cytokine processing, suggesting that NALP6 functions in immune cells to coordinate the transduction of pro-inflammatory signals.