Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Purification
ROCK1 Antibody is affinity chromatography purified via peptide column.
Immunogen
ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1. The immunogen is located within the first 50 amino acids of ROCK1.
ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
ROCK1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
ROCK1
(rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))
ROCK1 Antibody: ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.