SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Purification
SPP1 Antibody is affinity chromatography purified via peptide column.
Immunogen
SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1. The immunogen is located within amino acids 50 - 100 of SPP1.
SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
SPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
SPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).