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Collagen IV antibody (AA 1571-1669) (HRP)

COL4 Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (fro), IHC (p) Host: Rabbit Polyclonal HRP
Catalog No. ABIN707405
  • Target See all Collagen IV (COL4) Antibodies
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Binding Specificity
    • 16
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1571-1669
    Reactivity
    • 87
    • 42
    • 39
    • 22
    • 16
    • 10
    • 5
    • 2
    Human, Mouse, Rat, Pig
    Host
    • 56
    • 26
    • 12
    Rabbit
    Clonality
    • 67
    • 27
    Polyclonal
    Conjugate
    • 45
    • 10
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Collagen IV antibody is conjugated to HRP
    Application
    • 60
    • 44
    • 29
    • 29
    • 27
    • 26
    • 26
    • 23
    • 17
    • 9
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Cross-Reactivity
    Human, Mouse, Pig, Rat
    Predicted Reactivity
    Dog,Cow,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain
    Isotype
    IgG
    Top Product
    Discover our top product COL4 Primary Antibody
  • Application Notes
    WB 1:300-5000
    IHC-P 1:200-400
    IHC-F 1:100-500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Collagen IV (COL4) (Collagen, Type IV (COL4))
    Alternative Name
    Collagen 4 (COL4 Products)
    Synonyms
    collagen, type IV antibody, Let-2 antibody
    Background

    Synonyms: ICH, HANAC, POREN1, arresten, Collagen alpha-1(IV) chain, COL4A1

    Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

    Gene ID
    1282
    UniProt
    P02462
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