Osteopontin antibody
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- Target See all Osteopontin (SPP1) Antibodies
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Reactivity
- Rat
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This Osteopontin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Functional Studies (Func)
- Specificity
- Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
- Cross-Reactivity (Details)
- Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length rat Spp1 protein
- Clone
- OSP-4589
- Isotype
- IgG1
- Top Product
- Discover our top product SPP1 Primary Antibody
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- Application Notes
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Known_Application: Functional Studies, Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Bone. Found in plasma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Alternative Name
- SPP1 (SPP1 Products)
- Synonyms
- BNSP antibody, BSPI antibody, ETA-1 antibody, OPN antibody, 2AR antibody, Apl-1 antibody, Bsp antibody, Eta antibody, OP antibody, Opn antibody, Opnl antibody, Ric antibody, Spp-1 antibody, OSP antibody, zgc:111821 antibody, secreted phosphoprotein 1 antibody, SPP1 antibody, Spp1 antibody, spp1 antibody
- Background
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BNSP, Bone sialoprotein 1 (BSPI/BSP1), Early T lymphocyte activation 1 (ETA1), Nephropontin, immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), CALPHA1 fusion, Urinary stone protein, Uropontin,Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)
Cellular localisation: Secreted. - Molecular Weight
- 34.9kDa
- Gene ID
- 6696, 313
- UniProt
- P10451
- Pathways
- Regulation of Cell Size
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