HLA-E antibody (AA 33-261) (Biotin)

Catalog No. ABIN7155539

Product Details anti-HLA-E Antibody (Biotin)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Binding Specificity: AA 33-261

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-E antibody is conjugated to Biotin

Application: ELISA

Cross-Reactivity: Human

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human HLA class I histocompatibility antigen, alpha chain E protein (33-261AA)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Alternative Name: HLA-E (HLA-E Products)

Synonyms: EA1.2 antibody, EA2.1 antibody, HLA-6.2 antibody, MHC antibody, QA1 antibody, major histocompatibility complex, class I, E antibody, HLA-E antibody, PATR-E antibody

Background:

Background: Non-classical major histocompatibility class Ib molecule involved in immune self-nonself discrimination. In complex with B2M/beta-2-microglobulin binds nonamer self-peptides derived from the signal sequence of classical MHC class Ia molecules (VL9 peptides) (PubMed:9754572, PubMed:18083576, PubMed:18339401). Peptide-bound HLA-E-B2M heterotrimeric complex primarily functions as a ligand for natural killer (NK) cell inhibitory receptor KLRD1-KLRC1, enabling NK cells to monitor the expression of other MHC class I molecules in healthy cells and to tolerate self (PubMed:9754572, PubMed:9486650, PubMed:17179229, PubMed:18083576). Upon cellular stress, preferentially binds signal sequence-derived peptides from stress-induced chaperones and is no longer recognized by NK cell inhibitory receptor KLRD1-KLRC1, resulting in impaired protection from NK cells (PubMed:12461076). Binds signal sequence-derived peptides from non-classical MHC class Ib HLA-G molecules and acts as a ligand for NK cell activating receptor KLRD1-KLRC2, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal-fetal tolerance during pregnancy (PubMed:9754572, PubMed:30134159). Besides self-peptides, can also bind and present pathogen-derived peptides conformationally similar to VL9 peptides to alpha-beta T cell receptor (TCR) on unconventional CD8+ cytotoxic T cells, ultimately triggering antimicrobial immune response (PubMed:16474394, PubMed:30087334).

Aliases: HLA-E antibody, HLA-6.2 antibody, HLAEHLA class I histocompatibility antigen antibody, alpha chain E antibody, MHC class I antigen E) [Cleaved into: Soluble HLA class I histocompatibility antigen antibody, alpha chain E antibody, sHLA-E)] antibody

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process