CRBN antibody (AA 1-280)

Catalog No. ABIN7165198

Product Details anti-CRBN Antibody

Key Features:

• Rabbit anti-CRBN Antibody reliably detects CRBN in human species.
• Polyclonal, unconjugated antibody for ELISA, WB, IHC.
• Highly specific CRBN detection. See validation images.

Target: CRBN (Cereblon (CRBN))

Binding Specificity: AA 1-280

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRBN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human Protein cereblon protein (1-280AA)

Isotype: IgG

Product Specific Information:

What can the CRBN antibody ABIN7165198 be used for? This polyclonal, unconjugated rabbit anti-CRBN antibody is suitable for the detection of cereblon (CRBN) in ELISA, Western blotting and immunohistochemistry. The CRBN antibody has been validated for the human species. Based on the sequence, cross-reactivity with mouse can be assumed. Recombinant human cereblon protein was used as immunogen for production.

What validation data is available for this CRBN antibody? The CRBN antibody currently has three product images demonstrating its performance in IHC and Western blot. Use our high-quality CRBN Antibody to reliably detect Cereblon.

What is the function of CRBN? CRBN is a substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 or ILF2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. (UniProt)

Application Details

Application Notes: Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for CRBN

Target: CRBN (Cereblon (CRBN))

Alternative Name: CRBN (CRBN Products)

Synonyms: zgc:92404 antibody, mrt2a antibody, F3N11.21 antibody, MRT2 antibody, MRT2A antibody, 2610203G15Rik antibody, 2900045O07Rik antibody, AF229032 antibody, AW108261 antibody, piL antibody, cereblon antibody, ATP-dependent protease La (LON) domain protein antibody, crbn antibody, CRBN antibody, AT2G25740 antibody, Crbn antibody

Background:

Background: Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. May play a role in memory and learning by regulating the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. Binding of pomalidomide and other thalidomide-related drugs changes the substrate specificity of the human protein, leading to decreased degradation of MEIS2 and other target proteins and increased degradation of MYC, IRF4, IKZF1 and IKZF3.

Aliases: 2610203G15Rik antibody, 2900045O07Rik antibody, AF229032 antibody, AW108261 antibody, Cereblon antibody, Crbn antibody, CRBN_HUMAN antibody, DKFZp781K0715 antibody, MGC27358 antibody, MRT2A antibody, OTTHUMP00000209555 antibody, piL antibody, Protein cereblon antibody, Protein x 0001 antibody

UniProt: Q96SW2