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Osteopontin antibody (AA 230-310)

SPP1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7209325
  • Target See all Osteopontin (SPP1) Antibodies
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Binding Specificity
    • 15
    • 15
    • 15
    • 13
    • 12
    • 7
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 230-310
    Reactivity
    • 138
    • 71
    • 61
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 119
    • 48
    • 5
    • 4
    • 2
    Rabbit
    Clonality
    • 122
    • 49
    Polyclonal
    Conjugate
    • 85
    • 14
    • 13
    • 8
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Osteopontin antibody is un-conjugated
    Application
    • 143
    • 73
    • 47
    • 39
    • 39
    • 24
    • 20
    • 17
    • 14
    • 13
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    OPN Polyclonal Antibody
    Specificity
    OPN Polyclonal Antibody detects endogenous levels of OPN protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    Immunogen
    Synthesized peptide derived from the C-terminal region of human OPN at AA range: 230-310
    Isotype
    IgG
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    Discover our top product SPP1 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
    Comment

    Primary Antibody

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • Target
    Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
    Alternative Name
    OPN (SPP1 Products)
    Synonyms
    BNSP antibody, BSPI antibody, ETA-1 antibody, OPN antibody, 2AR antibody, Apl-1 antibody, Bsp antibody, Eta antibody, OP antibody, Opn antibody, Opnl antibody, Ric antibody, Spp-1 antibody, OSP antibody, zgc:111821 antibody, secreted phosphoprotein 1 antibody, SPP1 antibody, Spp1 antibody, spp1 antibody
    Background
    Rabbit Anti-OPN Polyclonal Antibody,SPP1, BNSP, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin,Secreted phosphoprotein 1 encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix. Secreted phosphoprotein 1 is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to secreted phosphoprotein 1. Secreted phosphoprotein 1 is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for SPP1.,Osteopontin
    Molecular Weight
    observerd band 60kDa
    Gene ID
    6696
    UniProt
    P10451
    Pathways
    Regulation of Cell Size
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