Osteopontin antibody (AA 230-310)
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- Target See all Osteopontin (SPP1) Antibodies
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Binding Specificity
- AA 230-310
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Osteopontin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- OPN Polyclonal Antibody
- Specificity
- OPN Polyclonal Antibody detects endogenous levels of OPN protein.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Immunogen
- Synthesized peptide derived from the C-terminal region of human OPN at AA range: 230-310
- Isotype
- IgG
- Top Product
- Discover our top product SPP1 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
- Comment
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Primary Antibody
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Target
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Alternative Name
- OPN (SPP1 Products)
- Synonyms
- BNSP antibody, BSPI antibody, ETA-1 antibody, OPN antibody, 2AR antibody, Apl-1 antibody, Bsp antibody, Eta antibody, OP antibody, Opn antibody, Opnl antibody, Ric antibody, Spp-1 antibody, OSP antibody, zgc:111821 antibody, secreted phosphoprotein 1 antibody, SPP1 antibody, Spp1 antibody, spp1 antibody
- Background
- Rabbit Anti-OPN Polyclonal Antibody,SPP1, BNSP, OPN, PSEC0156, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin,Secreted phosphoprotein 1 encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix. Secreted phosphoprotein 1 is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to secreted phosphoprotein 1. Secreted phosphoprotein 1 is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for SPP1.,Osteopontin
- Molecular Weight
- observerd band 60kDa
- Gene ID
- 6696
- UniProt
- P10451
- Pathways
- Regulation of Cell Size
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