Actin antibody

Catalog No. ABIN7211714

Product Details anti-Actin Antibody

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rabbit, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes actin isotypes α and γ of skeletal, cardiac and smooth muscle. This antibody labels rhabdomyosarcomas, hemangiopericytomas, leiomyosarcomas and leiomyomas but does not react with non-myogenic sarcomas. It is nonreactive with other neoplasms such as carcinoma or lymphoma and with other mesenchymal and all epithelial cells, with the exception of myoepithelium.

Characteristics: Muscle actin monoclonal antibody (HHF35)

Purification: Affinity purified.,Purified from hybridoma tissue culture supernatant.

Immunogen: Human myocardium (proteins extracted in 2% SDS).

Clone: HHF35

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections, this antibody can be used for staining of paraffin-embedded tissue sections fixed in neutral buffered formalin and is compatible with most commonly used histological fixatives. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection).General suggested starting dilution is 1:20-1:50.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. In PBS containing 0.1 % sodium azide and 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 °C.

Storage: -20 °C

Storage Comment: +4°C

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Muscle actin (ACTA1 Products)

Synonyms: ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, acta antibody, asma antibody, cftd antibody, cftd1 antibody, cftdm antibody, mpfd antibody, nem1 antibody, AA959943 antibody, Acta-2 antibody, Acts antibody, Actsk-1 antibody, actc antibody, actc1 antibody, cfk antibody, hm:zeh0631 antibody, zeh0631 antibody, acta1 antibody, hm:zeh0293 antibody, zgc:112098 antibody, actin, alpha 1, skeletal muscle antibody, actin, alpha 1, skeletal muscle L homeolog antibody, actin, alpha 1b, skeletal muscle antibody, actin, muscle antibody, actin, alpha skeletal muscle antibody, actin, alpha 1a, skeletal muscle antibody, ACTA1 antibody, acta1.L antibody, acta1 antibody, Acta1 antibody, acta1b antibody, LOC100533357 antibody, LOC109479779 antibody, LOC109108280 antibody, acta1a antibody

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development