IRS1 antibody (pSer636, pSer639) (HRP)
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- Target See all IRS1 Antibodies
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
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Binding Specificity
- pSer636, pSer639
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IRS1 antibody is conjugated to HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This phosphorylation site is homologous to Ser632 + ser635 in Mouse and Rat IRS1. This protein is known to be highly conserved in this sequences region, thus , this antibody may react with IRS2 when phosphorylated at Ser679 + Ser682 in Human , Ser675 + Ser678 in Mouse, and Ser676 +Ser679 in Rat based on homology.
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Dog,Cow,Pig,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of Ser636/639 [PM(p-S)PK(p-S)VS]
- Isotype
- IgG
- Top Product
- Discover our top product IRS1 Primary Antibody
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- Application Notes
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WB 1:300-5000
IHC-P 1:200-400 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IRS1 (Insulin Receptor Substrate 1 (IRS1))
- Alternative Name
- IRS1 + (IRS1 Products)
- Synonyms
- HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody
- Background
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Synonyms: HIRS-1, Insulin receptor substrate 1, IRS-1, IRS1
Background: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
- Gene ID
- 3667
- UniProt
- P35568
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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