c-MYC antibody (HRP)

Catalog No. ABIN920765

The Chicken Polyclonal anti-c-MYC antibody is suitable to detect c-MYC. It has been validated for WB and ELISA.

Quick Overview for c-MYC antibody (HRP) (ABIN920765)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Host: Chicken

Clonality: Polyclonal

Conjugate: This c-MYC antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Product Details anti-c-MYC Antibody (HRP)

Application Details

Application Notes: ELISA: Use at 1:10,000. Western blot: Use at 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2% BSA and 0.1% thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: Chickens were immunized with the C-Myc peptide conjugated with KLH. After multiple immunizations in Freund's adjuvant, eggs were collected. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid phase. The antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: Antibody specificity was verified by ELISA against the peptide conjugated to BSA. A 1:20,000 dilution of the antibody gave an OD = 1.0 in a 15 minute reaction using TMB as the substrate. Appropriate specificity controls were run.

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect