PC (C-Term) antibody
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- Target
- PC
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB)
- Purification
- Purified
- Immunogen
- PC antibody was raised in rabbit using the C terminal of PC as the immunogen
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- Application Notes
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WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator. - Comment
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PC Blocking Peptide, catalog no. 33R-8614, is also available for use as a blocking control in assays to test for specificity of this PC antibody
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C, following reconstitution, aliquot and store at -20 °C.
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- Target
- PC
- Background
- This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. Synonyms: Polyclonal PC antibody, Anti-PC antibody, pyruvate carboxylase antibody, PCB antibody.
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