Western Bloting: 1/500 - 1/2000. ELISA: Propose dilution 1/10000. Not yet tested in other applications. Determining optimal working dilutions by titration test.
Restrictions
For Research Use only
Format
Liquid
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Fentress: "Streams and patterns in behavior as challenges for future technologies." in: Behavior research methods, Vol. 41, Issue 3, pp. 765-71, (2009) (PubMed).
Duncan, Al-Attar, Rolland, Harper, Spendlove, Durrant: "Cytoplasmic p27 expression is an independent prognostic factor in ovarian cancer." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 29, Issue 1, pp. 8-18, (2009) (PubMed).
Lee, Kim: "The function of p27 KIP1 during tumor development." in: Experimental & molecular medicine, Vol. 41, Issue 11, pp. 765-71, (2009) (PubMed).
P27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originating from lung, breast, colon, ovary, esophagus, thyroid and prostate. Tissue specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, CDKN1B