This Estrogen Receptor alpha antibody is un-conjugated
Application
Immunohistochemistry (IHC)
Specificity
Estrogen Receptor- (Ab-118) antibody detects endogenous levels of total Estrogen Receptor- protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor- around the phosphorylation site of serine118 (Q-L-SP-P-F).
ESR1
Reactivity: Human, Mouse, Rat
WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Pentecost, Bradley, Gierthy, Ding, Fasco: "Gene regulation in an MCF-7 cell line that naturally expresses an estrogen receptor unable to directly bind DNA." in: Molecular and cellular endocrinology, Vol. 238, Issue 1-2, pp. 9-25, (2005) (PubMed).
Medunjanin, Hermani, De Servi, Grisouard, Rincke, Mayer: "Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity." in: The Journal of biological chemistry, Vol. 280, Issue 38, pp. 33006-14, (2005) (PubMed).
Masuhiro, Mezaki, Sakari, Takeyama, Yoshida, Inoue, Yanagisawa, Hanazawa, Omalley, Kato: "Splicing potentiation by growth factor signals via estrogen receptor phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 23, pp. 8126-31, (2005) (PubMed).