anti-Mouse (Murine) CD11b antibody for Fluorescence Microscopy

Recommended CD11b Antibody

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Reactivity
Mouse (Murine)
656
431
146
80
35
33
30
25
18
13
10
10
8
8
6
5
4
4
2
Host
Rat
475
345
175
16
6
2
Clonality
Monoclonal
Conjugate
This CD11b antibody is conjugated to PE
139
115
90
62
17
16
14
12
11
11
10
10
10
9
9
6
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP)
781
246
207
155
139
111
75
60
49
32
20
14
13
11
5
5
4
3
3
2
2
1
1
1
1
Options

Available images

Catalog No. ABIN6657853
$ 477.40
Plus shipping costs $45.00

General

Antigen Integrin alpha M (ITGAM) Antibodies
Reactivity Mouse (Murine)
(656), (431), (146), (80), (35), (33), (30), (25), (18), (13), (10), (10), (8), (8), (6), (5), (4), (4), (2)
Host Rat
(475), (345), (175), (16), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD11b antibody is conjugated to PE
(139), (115), (90), (62), (17), (16), (14), (12), (11), (11), (10), (10), (10), (9), (9), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Fluorescence Microscopy (FM), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(781), (246), (207), (155), (139), (111), (75), (60), (49), (32), (20), (14), (13), (11), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Supplier

Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling Images
Cross-Reactivity (Details) Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Purification Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b. . Cross-reactivity with CD11b from other sources has not been tested. Anti-CD11b is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Immunogen

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Clone M1-70
Isotype IgG2b
Labeling Ratio 1-2
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background

Synonyms: CD11b

Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gene Name: Itgam

UniProt A1E2I0
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling Images back to top
Application Notes

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD11b is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

IF Microscopy Dilution: User Optimized

Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details Images back to top
Format Liquid
Buffer

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha M (ITGAM) antibody (PE) (ABIN6657853) Flow Cytometry of anti-CD11b PE - 200-508-N79 Flow Cytometry of anti-CD11b Phycoeryth...
Immunofluorescence (IF) image for anti-Integrin alpha M (ITGAM) antibody (PE) (ABIN6657853) Immunofluorescence of anti-CD11b PE - 200-508-N79 Immunofluorescence Microscopy of An...