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|Antigen||CASP8 and FADD-Like Apoptosis Regulator (CFLAR) Antibodies|
|Epitope||C-Term, AA 191-209 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CFLAR antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
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Product Details anti-CFLAR AntibodyTarget Details CFLAR Application Details Handling References for anti-CFLAR antibody (ABIN318973) Images
|Specificity||This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).|
|Immunogen||A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.|
Target Details CFLARProduct Details anti-CFLAR Antibody Application Details Handling References for anti-CFLAR antibody (ABIN318973) Images back to top
|Alternative Name||CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)|
|Background||FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP|
|Pathways||Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development|
Application DetailsProduct Details anti-CFLAR Antibody Target Details CFLAR Handling References for anti-CFLAR antibody (ABIN318973) Images back to top
Immunohistochemistry on frozen sections: 2g/mL. Western Blot: 1: 1000, detects bands of approximately 35 kDa and 25 kDa in Jurkat celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CFLAR Antibody Target Details CFLAR Application Details References for anti-CFLAR antibody (ABIN318973) Images back to top
|Buffer||Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)|
|Precaution of Use||This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CFLAR antibody (ABIN318973)Product Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling Images back to top
Thome, Schneider, Hofmann, Fickenscher, Meinl, Neipel, Mattmann, Burns, Bodmer, Schröter, Scaffidi, Krammer, Peter, Tschopp: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997
Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997
Hu, Vincenz, Ni, Gentz, Dixit: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997
Irmler, Thome, Hahne, Schneider, Hofmann, Steiner, Bodmer, Schröter, Burns, Mattmann, Rimoldi, French, Tschopp: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997
ImagesProduct Details anti-CFLAR Antibody Target Details CFLAR Application Details Handling References for anti-CFLAR antibody (ABIN318973) back to top