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|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling Images
|Specificity||This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.|
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Purification||Protein G purified|
|Immunogen||Human vimentin recombinant protein|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Gene Symbol: VIM|
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
|Application Notes||Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLELISA (For coating, order NBP2-34809 (BSA free version of NBP2-32910))|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store at 4°C.|