anti-Chicken Vimentin antibody for Cytometry by Time of Flight

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
922
399
352
218
138
129
118
83
79
51
50
42
41
35
30
12
8
7
5
4
4
3
2
2
2
1
1
1
1
1
Host
Mouse
566
322
28
14
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
25
23
23
17
16
13
13
13
13
13
13
13
12
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
736
343
323
303
255
246
245
140
87
40
15
14
13
9
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN4365312
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365314 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 1
1 ABIN4365430 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
1 ABIN4365431 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(922), (399), (352), (218), (138), (129), (118), (83), (79), (51), (50), (42), (41), (35), (30), (12), (8), (7), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(566), (322), (28), (14), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (25), (23), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (12), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(736), (343), (323), (303), (255), (246), (245), (140), (87), (40), (15), (14), (13), (9), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images
Specificity V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Protein A or G purified
Immunogen Clone V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Research Area Lineage Markers, Cell/Tissue Markers, Cytoskeleton, Neural Stem Cell marker
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Application Notes Western Blot 1:100-1:1000, Flow Cytometry 1:25-1:200, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:200, Immunohistochemistry-Frozen 1:25-1:200, CyTOF-readyFor staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365312) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365312)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rigoutsos, Lee, Nam, Anfossi, Pasculli, Pichler, Jing, Rodriguez-Aguayo, Telonis, Rossi, Ivan, Catela Ivkovic, Fabris, Clark, Ling, Shimizu, Redis, Shah, Zhang, Okugawa, Jung, Tsirigos, Huang, Ferdin et al.: "N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. ..." in: Genome biology, Vol. 18, Issue 1, pp. 98, 2017

Hu, Ling, Xu, Han: "Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing." in: PLoS ONE, Vol. 9, Issue 3, pp. e92676, 2014 (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - Analysis of PE conjugate of NBP1-97670. An ...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - An intracellular stain was performed on Jur...