anti-Chicken Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Rat (Rattus), Chicken, Potoroo
1021
427
386
260
168
158
147
115
108
76
63
51
47
42
28
27
24
14
9
6
3
2
2
1
1
1
1
1
1
1
1
Host
Mouse
618
367
31
14
12
11
11
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
803
418
369
334
330
275
225
134
86
63
40
26
15
13
8
7
6
3
3
3
3
2
1
1
1
1
1
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Catalog No. ABIN335382
$ 383.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.88187 ABIN94492 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10 1
22.88187 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
16.88187 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
16.88187 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
16.132376 ABIN6254183 WB Goat IgG C-Term Log in to see Polyclonal 0
12.38187 ABIN5618631 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 0
10.750505 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
10 ABIN604756 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN6166931 FACS IF IHC (f) WB APC Mouse IgG1 Log in to see VM452 0
10 ABIN6166938 FACS IF IHC (f) WB PerCP Mouse IgG1 Log in to see VM452 0
10 ABIN6166939 FACS IF IHC (f) WB PE Mouse IgG1 Log in to see VM452 0
10 ABIN6166942 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
9.250505 ABIN223315 WB Rabbit IgG Log in to see Polyclonal 2
7 ABIN239818 ICC IHC IHC (p) IP WB Mouse IgM Log in to see VI-10 0
4 ABIN127264 IHC (p) IP WB Rabbit Log in to see Polyclonal 0
4 ABIN1724173 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10 0
4 ABIN3026900 FACS IF IHC (p) WB Mouse IgG1 Log in to see SPM576 0
4 ABIN2469991 ICC IP IHC WB Mouse IgM Log in to see VI-10 0
3.560627 ABIN4365312 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 2
1 ABIN112306 EIA IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see VIM 3B4 10

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Rat (Rattus), Chicken, Potoroo
(1021), (427), (386), (260), (168), (158), (147), (115), (108), (76), (63), (51), (47), (42), (28), (27), (24), (14), (9), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(618), (367), (31), (14), (12), (11), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(803), (418), (369), (334), (330), (275), (225), (134), (86), (63), (40), (26), (15), (13), (8), (7), (6), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335382) Images
Specificity Human, rat, chicken and potoroo.
Purification Purified
Immunogen V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN335382) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN335382) Images back to top
Application Notes V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN335382) Images back to top
Storage 4 °C

References for anti-Vimentin antibody (ABIN335382)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

De Visscher, Plusquin, Mesure, Flameng: "Selection of an immunohistochemical panel for cardiovascular research in sheep." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 18, Issue 4, pp. 382-91, 2010

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984

Background publications

Brussee, Smit, Koopman, Wijga, Kerkhof, Corver, Vos, Gerritsen, Grobbee, Brunekreef, Merkus, de Jongste: "Interrupter resistance and wheezing phenotypes at 4 years of age." in: American journal of respiratory and critical care medicine, Vol. 169, Issue 2, pp. 209-13, 2004

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335382) back to top
Supplier Images
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody (Image 2)
 image for anti-Vimentin (VIM) antibody (ABIN335382) anti-Vimentin (VIM) antibody (Image 3)