anti-Dog (Canine) Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken, Goat, Dog (Canine), Monkey, Zebrafish (Danio rerio)
1005
421
379
263
170
160
148
117
109
76
63
50
46
42
27
26
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Mouse
613
355
30
14
12
11
11
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
785
413
365
336
329
270
226
134
81
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
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Catalog No. ABIN335380
$ 447.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.255764 ABIN335381 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
19.802607 ABIN2783289 IHC WB Rabbit C-Term Log in to see Polyclonal 1
17.255764 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
17.255764 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
16.802607 ABIN2773915 IHC WB Rabbit N-Term Log in to see Polyclonal 1
16.55837 ABIN6254183 WB Goat IgG C-Term Log in to see Polyclonal 0
12.755764 ABIN5618631 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 0
12.755764 ABIN5618630 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV203 0
10 ABIN604756 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
10 ABIN6166931 FACS IF IHC (f) WB APC Mouse IgG1 Log in to see VM452 0
10 ABIN6166939 FACS IF IHC (f) WB PE Mouse IgG1 Log in to see VM452 0
10 ABIN6166938 FACS IF IHC (f) WB PerCP Mouse IgG1 Log in to see VM452 0
10 ABIN6166942 FACS IF IHC (f) WB Mouse IgG1 Log in to see VM452 0
7 ABIN1740711 ICC IF IHC (p) WB Chicken Log in to see Polyclonal 0
4.8026066 ABIN126914 WB Mouse IgG Log in to see 11H6 0
4.8026066 ABIN2560726 ELISA WB Goat IgG Log in to see Polyclonal 0
4 ABIN321407 WB Rabbit IgG AA 110-159 Log in to see Polyclonal 0
4 ABIN3026900 FACS IF IHC (p) WB Mouse IgG1 Log in to see SPM576 0
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN112354 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see RV202 5

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken, Goat, Dog (Canine), Monkey, Zebrafish (Danio rerio)
(1005), (421), (379), (263), (170), (160), (148), (117), (109), (76), (63), (50), (46), (42), (27), (26), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(613), (355), (30), (14), (12), (11), (11), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
(785), (413), (365), (336), (329), (270), (226), (134), (81), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335380) Images
Specificity Human, mouse, rat, hamster, chicken, goat, canine and monkey.
Purification Purified
Immunogen RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Clone RV202
Isotype IgG1
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN335380) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN335380) Images back to top
Application Notes RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN335380) Images back to top
Storage 4 °C

References for anti-Vimentin antibody (ABIN335380)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN335380) back to top
Supplier Images
 image for anti-Vimentin (VIM) antibody (ABIN335380) anti-Vimentin (VIM) antibody
 image for anti-Vimentin (VIM) antibody (ABIN335380) anti-Vimentin (VIM) antibody (Image 2)