anti-Human Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Epitope
AA 435-466, C-Term
27
26
23
22
19
18
16
16
15
13
8
7
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1006
421
379
266
173
163
152
119
112
76
62
51
47
42
28
26
24
14
9
6
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
616
354
28
14
12
11
11
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
31
26
25
24
16
15
12
12
12
12
12
12
12
12
9
9
7
7
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
785
414
364
335
330
270
227
135
81
63
40
26
15
13
8
7
7
4
3
3
3
1
1
1
1
1
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Catalog No. ABIN3042344
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0645456 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 19
3.0645456 ABIN6269441 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 14
3.0645456 ABIN672786 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 416-466 Log in to see Polyclonal 29
3.0645456 ABIN4365306 ICC IF IHC IHC (p) SimWes WB Rabbit IgG Log in to see Polyclonal 3
3.0645456 ABIN2773915 IHC WB Rabbit N-Term Log in to see Polyclonal 1
3.0645456 ABIN2783289 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.0645456 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
3.0645456 ABIN441334 ICC IF IHC IHC (fro) IHC (p) IP WB Rabbit IgG Center Log in to see Polyclonal 2
3.0645456 ABIN1109485 IF WB Mouse IgG2b pSer55 Log in to see 4A4 11
3.0645456 ABIN2854814 ICC IHC (fro) IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.0645456 ABIN223315 WB Rabbit IgG Log in to see Polyclonal 2
3.0645456 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
3.0645456 ABIN1881993 FACS IF IHC WB Rabbit Log in to see Polyclonal 3
3.0645456 ABIN1449160 IF WB Rat IgG2a pSer50 Log in to see TM50 7
3.0645456 ABIN389211 IF IHC (p) WB Rabbit Ig Fraction AA 63-90, Ser82 Log in to see Polyclonal 3
3.0645456 ABIN389212 FACS IF IHC (p) WB Rabbit Ig Fraction AA 430-457, C-Term Log in to see Polyclonal 4
3.0645456 ABIN6265980 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 3
3.0645456 ABIN394656 IF IP WB Mouse IgG2a kappa AA 41-141 Log in to see 3E9 5
3.0645456 ABIN3223017 IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.0645456 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Vimentin (VIM) Antibodies
Epitope AA 435-466, C-Term
(27), (26), (23), (22), (19), (18), (16), (16), (15), (13), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1006), (421), (379), (266), (173), (163), (152), (119), (112), (76), (62), (51), (47), (42), (28), (26), (24), (14), (9), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(616), (354), (28), (14), (12), (11), (11), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(35), (31), (26), (25), (24), (16), (15), (12), (12), (12), (12), (12), (12), (12), (12), (9), (9), (7), (7), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(785), (414), (364), (335), (330), (270), (227), (135), (81), (63), (40), (26), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 56 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images
Purpose Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Sequence DTHSKRTLLI KTVETRDGQV INETSQHHDD LE
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vimentin
Protein Name: Vimentin
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.
Isotype IgG
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
Antigen
Alternative Name VIM (VIM Antibody Abstract)
Background VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Synonyms: CTRCT30 antibody|Epididymis luminal protein 113 antibody|FLJ36605 antibody|HEL113 antibody|VIM antibody|VIME_HUMAN antibody|vimentin antibody
Gene ID 7431
UniProt P08670
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Vimentin is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN3042344) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Vimentin antibody (ABIN3042344)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Quan, Du, Hou, Wang, Zhang: "Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma." in: Oncology reports, Vol. 38, Issue 2, pp. 850-858, 2017

Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." in: BioMed research international, Vol. 2015, pp. 871202, 2015

Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, 2015

Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, 2015

Zhang, Wang, Jiang, Niu, Fu, Luo, Cooper, Smith, He: "Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro." in: Experimental cell research, Vol. 332, Issue 2, pp. 259-66, 2015

Bao, Wang, Ma, Wang, Un Nesa, Wang, Wang, Jia, Wang, Tian, Cheng: "Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma." in: Biochemical and biophysical research communications, Vol. 458, Issue 2, pp. 441-7, 2015

Jiang, Lv, Wang, Wang, Sun, Jia, Wang, Song, Ni: "Activation of the NLRP3/caspase-1 inflammasome in human dental pulp tissue and human dental pulp fibroblasts." in: Cell and tissue research, Vol. 361, Issue 2, pp. 541-55, 2015

Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." in: Stem cell research & therapy, Vol. 6, pp. 22, 2015

Zhang, Wang, Yang, Shi, Gan: "?2-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro." in: BMC nephrology, Vol. 16, pp. 60, 2015

Xia, Yin, Jiang, Guo, Tian, Jiang, Wu, Chen, Wu, He: "Downregulating HMGA2 attenuates epithelial-mesenchymal transition-induced invasion and migration in nasopharyngeal cancer cells." in: Biochemical and biophysical research communications, Vol. 463, Issue 3, pp. 357-63, 2015

Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014

Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, 2014

Duan, Lin, Li, Ding, Qian, Zhang, Ge, Fan, Li: "Effects of inhibition of hedgehog signaling on cell growth and migration of uveal melanoma cells." in: Cancer biology & therapy, Vol. 15, Issue 5, pp. 544-59, 2014

Zhang, Shi, Li, Zhang, Hao: "Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 87-93, 2014

Han, Fu, Luo, He, Liu, Liao, Tian, Yan: "BVES inhibition triggers epithelial-mesenchymal transition in human hepatocellular carcinoma." in: Digestive diseases and sciences, Vol. 59, Issue 5, pp. 992-1000, 2014

Gai, Lu, Tu, Liang, Zheng: "Caveolin-1 is up-regulated by GLI1 and contributes to GLI1-driven EMT in hepatocellular carcinoma." in: PLoS ONE, Vol. 9, Issue 1, pp. e84551, 2014

Yang, Lü, Hong, Xi, Zhang: "The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function." in: Biomaterials, Vol. 35, Issue 24, pp. 6195-205, 2014

Em, Kataria, Shah, Yadav: "A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos." in: Cytotechnology, 2014

Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, 2014

Wang, Feng, Zeng, Fan, Wu, Li, Liu, Huang, Huang, Yu, Yang: "Lipopolysaccharide (LPS)-induced autophagy is involved in the restriction of Escherichia coli in peritoneal mesothelial cells." in: BMC microbiology, Vol. 13, pp. 255, 2014

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN3042344) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody (ABIN3042344) Anti- Vimentin Picoband antibody, Western blotting All lanes: Anti Vimentin at 0.5ug...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody (ABIN3042344) Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Mouse Cardiac Muscle Tissue
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody (ABIN3042344) Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody (ABIN3042344) Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Rat Cardiac Muscle Tissue
Western Blotting (WB) image for anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody (ABIN3042344) Anti- Vimentin Picoband antibody, Western blottingAll lanes: Anti Vimentin at 0.5ug/...