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|Antigen||Vimentin (VIM) Antibodies|
|Epitope||AA 435-466, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|56 references available|
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images
|Purpose||Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Sequence||DTHSKRTLLI KTVETRDGQV INETSQHHDD LE|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vimentin
Protein Name: Vimentin
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.|
|Plasmids, Primers & others|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
|Alternative Name||VIM (VIM Antibody Abstract)|
VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Synonyms: CTRCT30 antibody|Epididymis luminal protein 113 antibody|FLJ36605 antibody|HEL113 antibody|VIM antibody|VIME_HUMAN antibody|vimentin antibody
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Vimentin is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN3042344) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-Vimentin antibody (ABIN3042344)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
|Product cited in:||
Quan, Du, Hou, Wang, Zhang: "Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma." in: Oncology reports, Vol. 38, Issue 2, pp. 850-858, 2017
Shen, Lu, Li, Zhang, Zhu: "Synthesis and characterization of biodegradable polyurethane for hypopharyngeal tissue engineering." in: BioMed research international, Vol. 2015, pp. 871202, 2015
Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, 2015
Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, 2015
Zhang, Wang, Jiang, Niu, Fu, Luo, Cooper, Smith, He: "Nuclear Factor I-C promotes proliferation and differentiation of apical papilla-derived human stem cells in vitro." in: Experimental cell research, Vol. 332, Issue 2, pp. 259-66, 2015
Bao, Wang, Ma, Wang, Un Nesa, Wang, Wang, Jia, Wang, Tian, Cheng: "Irradiated fibroblasts promote epithelial-mesenchymal transition and HDGF expression of esophageal squamous cell carcinoma." in: Biochemical and biophysical research communications, Vol. 458, Issue 2, pp. 441-7, 2015
Jiang, Lv, Wang, Wang, Sun, Jia, Wang, Song, Ni: "Activation of the NLRP3/caspase-1 inflammasome in human dental pulp tissue and human dental pulp fibroblasts." in: Cell and tissue research, Vol. 361, Issue 2, pp. 541-55, 2015
Su, Liu, Ma, Wang, E, Liu: "Enhancement of periodontal tissue regeneration by transplantation of osteoprotegerin-engineered periodontal ligament stem cells." in: Stem cell research & therapy, Vol. 6, pp. 22, 2015
Zhang, Wang, Yang, Shi, Gan: "?2-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro." in: BMC nephrology, Vol. 16, pp. 60, 2015
Xia, Yin, Jiang, Guo, Tian, Jiang, Wu, Chen, Wu, He: "Downregulating HMGA2 attenuates epithelial-mesenchymal transition-induced invasion and migration in nasopharyngeal cancer cells." in: Biochemical and biophysical research communications, Vol. 463, Issue 3, pp. 357-63, 2015
Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014
Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, 2014
Duan, Lin, Li, Ding, Qian, Zhang, Ge, Fan, Li: "Effects of inhibition of hedgehog signaling on cell growth and migration of uveal melanoma cells." in: Cancer biology & therapy, Vol. 15, Issue 5, pp. 544-59, 2014
Zhang, Shi, Li, Zhang, Hao: "Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 87-93, 2014
Han, Fu, Luo, He, Liu, Liao, Tian, Yan: "BVES inhibition triggers epithelial-mesenchymal transition in human hepatocellular carcinoma." in: Digestive diseases and sciences, Vol. 59, Issue 5, pp. 992-1000, 2014
Gai, Lu, Tu, Liang, Zheng: "Caveolin-1 is up-regulated by GLI1 and contributes to GLI1-driven EMT in hepatocellular carcinoma." in: PLoS ONE, Vol. 9, Issue 1, pp. e84551, 2014
Yang, Lü, Hong, Xi, Zhang: "The molecular mechanism for effects of TiN coating on NiTi alloy on endothelial cell function." in: Biomaterials, Vol. 35, Issue 24, pp. 6195-205, 2014
Em, Kataria, Shah, Yadav: "A comparative study on efficiency of adult fibroblasts and amniotic fluid-derived stem cells as donor cells for production of hand-made cloned buffalo (Bubalus bubalis) embryos." in: Cytotechnology, 2014
Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, 2014
Wang, Feng, Zeng, Fan, Wu, Li, Liu, Huang, Huang, Yu, Yang: "Lipopolysaccharide (LPS)-induced autophagy is involved in the restriction of Escherichia coli in peritoneal mesothelial cells." in: BMC microbiology, Vol. 13, pp. 255, 2014
ImagesProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN3042344) back to top