anti-Human Vimentin antibody for Western Blotting

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Epitope
N-Term
40
26
24
22
18
18
15
15
13
8
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
922
399
351
219
137
130
118
83
79
51
50
41
41
35
30
11
7
7
5
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
567
321
28
14
12
9
9
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
25
23
23
17
16
13
13
13
13
13
13
13
12
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
736
344
323
304
255
247
246
141
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2773915
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0817053 ABIN2783289 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.0817053 ABIN223315 WB Rabbit IgG Log in to see Polyclonal 2
3.0817053 ABIN1532790 IHC ELISA WB Rabbit IgG AA 31-80 Log in to see Polyclonal 2
3.0817053 ABIN1531760 IHC ELISA WB Rabbit IgG AA 31-80, pSer56 Log in to see Polyclonal 2
3.0817053 ABIN1533459 IHC ELISA WB Rabbit IgG AA 411-460 Log in to see Polyclonal 1
3.0817053 ABIN1109485 IF WB Mouse IgG2b pSer55 Log in to see 4A4 11
3.0817053 ABIN1449160 IF WB Rat IgG2a pSer50 Log in to see TM50 7
3.0817053 ABIN3042344 IHC (p) WB Rabbit IgG AA 435-466, C-Term Log in to see Polyclonal 56
3.0817053 ABIN1109484 IF WB Rat IgG2a Log in to see TM38 3
3.0817053 ABIN1109486 IF WB Rat IgG2a Log in to see TM71 9
3.0817053 ABIN1109487 IF WB Mouse IgG2b Log in to see MO82 14
3.0817053 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 18
3.0817053 ABIN2705329 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
3.0817053 ABIN1449152 IF WB Mouse IgG1 pSer33 Log in to see YT33 2
3.0817053 ABIN389211 IF IHC (p) WB Rabbit Ig AA 63-90, Ser82 Log in to see Polyclonal 3
3.0817053 ABIN389212 FACS IF IHC (p) WB Rabbit Ig AA 430-457, C-Term Log in to see Polyclonal 4
3.0817053 ABIN389213 FACS IHC (p) WB Rabbit Ig AA 152-181, Center Log in to see Polyclonal 3
3.0817053 ABIN672786 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 416-466 Log in to see Polyclonal 27
3.0817053 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
3.0817053 ABIN1532789 ELISA WB Rabbit IgG AA 56-105 Log in to see Polyclonal

General

Antigen Vimentin (VIM) Antibodies
Epitope N-Term
(40), (26), (24), (22), (18), (18), (15), (15), (13), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(922), (399), (351), (219), (137), (130), (118), (83), (79), (51), (50), (41), (41), (35), (30), (11), (7), (7), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(567), (321), (28), (14), (12), (9), (9), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (25), (23), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (12), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(736), (344), (323), (304), (255), (247), (246), (141), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN2773915) Images
Blocking Peptide Blocking peptide for this product available: ABIN986070
Sequence LNDRFANYID KVRFLEQQNK ILLAELEQLK GQGKSRLGDL YEEEMRELRR
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against VIM. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human VIM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN2773915) Images back to top
Antigen
Alternative Name VIM (VIM Antibody Abstract)
Background Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ36605
Protein Interaction Partner: PKN1, PPL, PLA2G2A, PA2G4, KRT20, TXLNB, CWF19L2, PNMA5, UBC, TP53, CHFR, SPRTN, SUMO2, SUMO3, TRIM68, STAU1, ASB15, ASB9, ASB16, ASB2, RNF2, EEF1D, SERPINH1, COPS8, FOXK1, YWHAQ, PARK2, FBXO6, CASP7, CASP3, KAT7, UBD, PINK1, BAG3, GSK3B, PAN2, CAPN1, SVI
Protein Size: 466
Molecular Weight 54 kDa
Gene ID 7431
NCBI Accession NM_003380, NP_003371
UniProt P08670
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN2773915) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 466 AA

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN2773915) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN2773915)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Garcia-Verdugo, Synguelakis, Degrouard, Franco, Valot, Zivy, Chaby, Tanfin: "Interaction of surfactant protein A with the intermediate filaments desmin and vimentin." in: Biochemistry, Vol. 47, Issue 18, pp. 5127-38, 2008

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN2773915) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin (VIM) (N-Term) antibody (ABIN2773915) anti-Vimentin (VIM) (N-Term) antibody
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) (N-Term) antibody (ABIN2773915) anti-Vimentin (VIM) (N-Term) antibody (Image 2)