anti-Mouse (Murine) Vimentin antibody for Simple Western

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • CTRCT30
  • cb28
  • vime
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • vimentin L homeolog
  • VIM
  • Vim
  • vim
  • vim.L
Reactivity
Human, Mouse (Murine)
1039
463
415
276
179
169
157
117
116
74
59
54
50
47
42
30
25
12
7
6
3
2
2
1
1
1
1
1
Host
Rabbit
658
346
29
14
12
11
10
5
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
35
32
30
28
28
18
17
16
14
14
14
14
14
14
14
9
9
8
8
7
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
824
431
397
371
338
273
266
160
87
63
40
27
15
13
8
7
7
4
3
3
3
1
1
1
1
1
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Available images

Catalog No. ABIN4365306
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365308 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1 0
1 ABIN4365379 ICC IF SimWes WB Rabbit Log in to see Polyclonal 1
1 ABIN4365307 ICC IF IHC SimWes WB Mouse IgG2a kappa Log in to see 2D1 0
1 ABIN4365378 ICC IF SimWes WB Rabbit Log in to see Polyclonal 0

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Mouse (Murine)
(1039), (463), (415), (276), (179), (169), (157), (117), (116), (74), (59), (54), (50), (47), (42), (30), (25), (12), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(658), (346), (29), (14), (12), (11), (10), (5)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(35), (32), (30), (28), (28), (18), (17), (16), (14), (14), (14), (14), (14), (14), (14), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(824), (431), (397), (371), (338), (273), (266), (160), (87), (63), (40), (27), (15), (13), (8), (7), (7), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365306) Images
Specificity Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Purification Immunogen affinity purified
Immunogen This antibody was developed against Recombinant Protein corresponding to amino acids:FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVES
Isotype IgG
Plasmids, Primers & others

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365306) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365306) Images back to top
Application Notes Western Blot 1:100-1:500, Simple Western 1:20, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1 - 4 μg/mL, Immunohistochemistry-Paraffin 1:500-1:1000Immunohistochemistry retrieval recommended: HIER pH 6, Immunocytochemistry/Immunofluorescence Fixation Permeabilization: PFA/Triton X-100. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365306) Images back to top
Format Liquid
Buffer PBS ( pH 7.2) and 40 % Glycerol
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365306)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Lopez-Rodriguez, Acaz-Fonseca, Viveros, Garcia-Segura: "Changes in cannabinoid receptors, aquaporin 4 and vimentin expression after traumatic brain injury in adolescent male mice. Association with edema and neurological deficit." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128782, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yamasaki, Seki, Yamada, Yoshino, Hidaka, Chiyomaru, Nohata, Kinoshita, Nakagawa, Enokida: "Tumor suppressive microRNA‑138 contributes to cell migration and invasion through its targeting of vimentin in renal cell carcinoma." in: International journal of oncology, Vol. 41, Issue 3, pp. 805-17, 2012

Stadler, Hjelmare, Neumann, Jonasson, Pepperkok, Uhlén, Lundberg: "Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy." in: Journal of proteomics, Vol. 75, Issue 7, pp. 2236-51, 2012

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365306) back to top
Supplier Images
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN4365306) Western Blot: Vimentin Antibody - Analysis in human cell line HeLa.
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN4365306) Western Blot: Vimentin Antibody - Analysis in mouse cell line NIH-3T3, rat cell line...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Vimentin (VIM) antibody (ABIN4365306) Immunohistochemistry-Paraffin: Vimentin Antibody - Staining of human rectum shows st...
Immunofluorescence (IF) image for anti-Vimentin (VIM) antibody (ABIN4365306) Immunocytochemistry/Immunofluorescence: Vimentin Antibody - Immunofluorescent staini...
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN4365306) Western Blot: Vimentin Antibody - Analysis in human cell lines U-251MG and MCF-7 usi...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Vimentin (VIM) antibody (ABIN4365306) Immunocytochemistry/Immunofluorescence: Vimentin Antibody - Staining of human cell l...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) antibody (ABIN4365306) Immunohistochemistry-Paraffin: Vimentin Antibody - Staining in human ovary and skele...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vimentin (VIM) antibody (ABIN4365306) Immunohistochemistry-Paraffin: Vimentin Antibody - Staining of human ovary shows hig...