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|Antigen||Vimentin (VIM) Antibodies|
|Epitope||AA 435-466, C-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|86 references available|
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images
|Purpose||Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.|
|Sequence||DTHSKRTLLI KTVETRDGQV INETSQHHDD LE|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Vimentin(VIM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: vimentin
Protein Name: Vimentin
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Vimentin (435-466aa DTHSKRTLLIKTVETRDGQVINETSQHHDDLE), identical to the related mouse and rat sequences.|
|Plasmids, Primers & others|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
|Alternative Name||VIM (VIM Antibody Abstract)|
VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Synonyms: CTRCT30 antibody|Epididymis luminal protein 113 antibody|FLJ36605 antibody|HEL113 antibody|VIM antibody|VIME_HUMAN antibody|vimentin antibody
|Pathways||Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN3042344) Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for Vimentin is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN3042344) Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
References for anti-Vimentin antibody (ABIN3042344)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
|Product cited in:||
Li, Zang, He, Chen, Yu, Pei, Hou, An, Yang, Zhang, Liu: "Expression of pannexin 1 and 2 in cortical lesions from intractable epilepsy patients with focal cortical dysplasia." in: Oncotarget, Vol. 8, Issue 4, pp. 6883-6895, 2018
Xu, Xiao, Luo, Chen, Zhang, Tao, Jiang, Chen, Shen: "Inhibitory effects of oxymatrine on TGF‑β1‑induced proliferation and abnormal differentiation in rat cardiac fibroblasts via the p38MAPK and ERK1/2 signaling pathways." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 5354-5362, 2018
Bu, Zhang, Wang, Lai: "Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest." in: International journal of oncology, Vol. 51, Issue 5, pp. 1405-1414, 2018
Gu, Fang, Kang, Hu, Yu, Li, Cheng, Gao: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, 2018
Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, 2018
Huang, Zheng, Pan, Wu, Su, Li, Wu, Sun: "Oral exposure of low-dose bisphenol A promotes proliferation of dorsolateral prostate and induces epithelial-mesenchymal transition in aged rats." in: Scientific reports, Vol. 8, Issue 1, pp. 490, 2018
Ling, Zhou, Zhou, Dai, Wu, Mi, Mao, Chen: "Lin28/microRNA-let-7a promotes metastasis under circumstances of hyperactive Wnt signaling in esophageal squamous cell carcinoma." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5265-5271, 2018
Jia, Jiang, Liu, Wang, Zhu, Zhu, Liu, Zhong, Xie, Huang, Jia, Li, Liu, Zuo, Cheng, Dai, Ren: "Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells." in: International journal of oncology, Vol. 52, Issue 6, pp. 1787-1800, 2018
Zhang, Nguyen, Shi, Burrell, Cullen, Le: "3D bio-printed scaffold-free nerve constructs with human gingiva-derived mesenchymal stem cells promote rat facial nerve regeneration." in: Scientific reports, Vol. 8, Issue 1, pp. 6634, 2018
Wang, Xu, Fu, Chen, Yang: "The Antihelminthic Niclosamide Inhibits Cancer Stemness, Extracellular Matrix Remodeling, and Metastasis through Dysregulation of the Nuclear β-catenin/c-Myc axis in OSCC." in: Scientific reports, Vol. 8, Issue 1, pp. 12776, 2018
Quan, Du, Hou, Wang, Zhang: "Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma." in: Oncology reports, Vol. 38, Issue 2, pp. 850-858, 2017
Guo, Yu, Xu, Lian, Yu, Cui, Ji, Chen, Li, Liu, Chen: "PAC1R agonist maxadilan enhances hADSC viability and neural differentiation potential." in: Journal of cellular and molecular medicine, Vol. 20, Issue 5, pp. 874-90, 2017
Deng, Li, Lin, Huang, Zeng, Wang, Li, Jin, Zhang, Li, Chen, Wang, Huang, Shao, Xu, Mao: "P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3." in: Theranostics, Vol. 6, Issue 2, pp. 204-18, 2017
Zheng, Zou, Shen, Xu, Wang, Fu, Fan: "Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth." in: Stem cells translational medicine, Vol. 5, Issue 9, pp. 1216-28, 2017
Yu, Liu, Liang, Ochs, Chen, Chen, Du, Tang, Huang, Du, Jin: "Sulfur Dioxide Protects Against Collagen Accumulation in Pulmonary Artery in Association With Downregulation of the Transforming Growth Factor β1/Smad Pathway in Pulmonary Hypertensive Rats." in: Journal of the American Heart Association, Vol. 5, Issue 10, 2017
Wang, Tu, Zhang, Xu, Qian: "Stellate Cell Activation and Imbalanced Expression of TGF-β1/TGF-β3 in Acute Autoimmune Liver Lesions Induced by ConA in Mice." in: BioMed research international, Vol. 2017, pp. 2540540, 2017
Wei, Zhu, Xiang, Song, Guo, Lin, Li, Zeng: "Corticosterone suppresses IL-1β-induced mPGE2 expression through regulation of the 11β-HSD1 bioactivity of synovial fibroblasts in vitro." in: Experimental and therapeutic medicine, Vol. 13, Issue 5, pp. 2161-2168, 2017
Wang, An, Yu, Zhang, Tang, Zhang, Li: "MiR-29b/TET1/ZEB2 signaling axis regulates metastatic properties and epithelial-mesenchymal transition in breast cancer cells." in: Oncotarget, Vol. 8, Issue 60, pp. 102119-102133, 2017
Wu, Wang, Ji, Ou, Xia, Zhang, Zhao: "C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells." in: American journal of translational research, Vol. 9, Issue 12, pp. 5708-5718, 2017
Deng, Guo, Liu, Zeng, Ning, Pei, Li, Chen, Guo, Li, Han, Xu: "Blocking protein phosphatase 2A signaling prevents endothelial-to-mesenchymal transition and renal fibrosis: a peptide-based drug therapy." in: Scientific reports, Vol. 6, pp. 19821, 2016
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