anti-Dog (Canine) CDKN1B antibody for Enzyme Immunoassay

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
C-Term
80
57
47
37
29
28
25
20
19
19
19
19
17
16
14
13
10
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
781
423
358
182
40
24
18
8
8
7
3
3
2
1
1
1
Host
Goat
474
306
38
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
40
35
35
34
33
29
9
7
7
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
632
338
242
233
200
197
101
94
84
54
37
17
12
5
5
3
1
1
1
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Catalog No. ABIN5552643
$ 462.00
Plus shipping costs $45.00

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope C-Term
(80), (57), (47), (37), (29), (28), (25), (20), (19), (19), (19), (19), (17), (16), (14), (13), (10), (8), (7), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(781), (423), (358), (182), (40), (24), (18), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Goat
(474), (306), (38), (2), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1B antibody is un-conjugated
(40), (35), (35), (34), (33), (29), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(632), (338), (242), (233), (200), (197), (101), (94), (84), (54), (37), (17), (12), (5), (5), (3), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Sequence C-EQTPKKPGLR RRQT
Specificity This antibody detects P27Kip1 / CDKN1B at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Cow, Canine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Immunogen Peptide from C Terminus of the protein sequence according to NP_004055.1 Genename: CDKN1B
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name CDKN1B / KIP1 (CDKN1B Antibody Abstract)
Background Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1, while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1
Gene ID 1027
NCBI Accession NP_004055
UniProt P46527
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 26 kDa band observed in Human Breast and Prostatelysates (calculated MW of 22.6 kDa according to NP_004055.1). The observed molecularweight corresponds to earlier findings in literature with different antibodies (Xia et al, ProcNatl Acad Sci U S A.2005 Sep 27,102(39): 14028-33, PMID: 16169901).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.