anti-Human CDKN1B antibody for Dot Blot

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
pThr157
82
55
47
42
36
27
24
20
19
19
19
19
17
16
14
13
13
10
9
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
789
439
375
182
42
24
18
8
8
7
3
3
2
1
1
1
Host
Rabbit
480
307
37
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
41
36
35
34
34
29
9
8
8
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
Application
Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
629
338
243
233
200
198
100
98
84
55
39
28
14
7
5
3
1
1
1
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Catalog No. ABIN389616
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.368652 ABIN389912 DB Rabbit Ig Fraction pThr198 Log in to see Polyclonal 3
27.368652 ABIN1881623 DB Rabbit pThr170 Log in to see Polyclonal 4
27.368652 ABIN1881621 DB Rabbit pSer140 Log in to see Polyclonal 4
27.368652 ABIN1881622 DB Rabbit pSer83 Log in to see Polyclonal 4
27.368652 ABIN3030632 DB ELISA Rabbit Ig Fraction pThr170 Log in to see Polyclonal 0
27.368652 ABIN3030630 DB ELISA Rabbit Ig Fraction pSer83 Log in to see Polyclonal 0
27.368652 ABIN3030631 DB IHC ELISA Rabbit Ig Fraction pThr157 Log in to see Polyclonal 0
27.368652 ABIN3002096 DB Rabbit IgG pSer140 Log in to see Polyclonal 0
27.368652 ABIN3002097 DB Rabbit IgG pSer83 Log in to see Polyclonal 0
27.368652 ABIN3002098 DB Rabbit IgG pThr170 Log in to see Polyclonal 0
27.368652 ABIN3001857 DB Rabbit IgG pThr198 Log in to see Polyclonal 0
27.368652 ABIN2970965 DB IHC Rabbit IgG pThr157 Log in to see Polyclonal 0
1 ABIN933144 DB WB Mouse IgG1 Log in to see 2144C8a 0
1 ABIN1832091 DB WB Mouse IgG1 Log in to see 2144C8a 0
1 ABIN2326922 DB ELISA Rabbit IgG pSer83 Log in to see Polyclonal 0
1 ABIN2326899 DB ELISA Rabbit IgG pSer140 Log in to see Polyclonal 0
1 ABIN2326959 DB ELISA Rabbit IgG pThr198 Log in to see Polyclonal 0
1 ABIN2326729 DB ELISA Rabbit IgG pThr170 Log in to see Polyclonal 0
1 ABIN1953077 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr198 Log in to see Polyclonal 0
1 ABIN1953949 DB ELISA APC Rabbit IgG pThr198 Log in to see Polyclonal 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope pThr157
(82), (55), (47), (42), (36), (27), (24), (20), (19), (19), (19), (19), (17), (16), (14), (13), (13), (10), (9), (8), (7), (7), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(789), (439), (375), (182), (42), (24), (18), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Rabbit
(480), (307), (37), (2), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This CDKN1B antibody is un-conjugated
(41), (36), (35), (34), (34), (29), (9), (8), (8), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(629), (338), (243), (233), (200), (198), (100), (98), (84), (55), (39), (28), (14), (7), (5), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN389616) Images
Specificity This Phospho-p27Kip1-T157 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1.
Clone RB28265
Isotype Ig Fraction
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling References for anti-CDKN1B antibody (ABIN389616) Images back to top
Antigen
Alternative Name p27Kip1 (CDKN1B Antibody Abstract)
Background P27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1,
Molecular Weight 22073 DA
Gene ID 1027
UniProt P46527
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling References for anti-CDKN1B antibody (ABIN389616) Images back to top
Application Notes DB = 1:500, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details References for anti-CDKN1B antibody (ABIN389616) Images back to top
Format Liquid
Concentration 0.38 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-CDKN1B antibody (ABIN389616)

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling Images back to top
Product cited in:

Le, Mao, He, Claret, Xia, Ahmed, Hung, Siddik, Bast: "The role of p27(Kip1) in dasatinib-enhanced paclitaxel cytotoxicity in human ovarian cancer cells." in: Journal of the National Cancer Institute, Vol. 103, Issue 18, pp. 1403-22, 2011 Method employed by authors: Western Blotting (WB)

Background publications

Wingate, Zhang, McGarhen, Bedrosian, Harper, Keyomarsi: "The tumor-specific hyperactive forms of cyclin E are resistant to inhibition by p21 and p27." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 15148-57, 2005

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Rassidakis, Feretzaki, Atwell, Grammatikakis, Lin, Lai, Claret, Medeiros, Amin: "Inhibition of Akt increases p27Kip1 levels and induces cell cycle arrest in anaplastic large cell lymphoma." in: Blood, Vol. 105, Issue 2, pp. 827-9, 2005

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling References for anti-CDKN1B antibody (ABIN389616) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr157) antibody (ABIN389616) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr157) antibody (ABIN389616) The anti-Phospho-p27Kip1-T157 Pab (ABIN389616) is used in Western blot to detect Phos...
Dot Blot (DB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr157) antibody (ABIN389616) Dot blot analysis of anti-p27Kip1-T157 Phospho-specific Pab (ABIN389616) on nitrocell...