anti-Monkey CDKN1B antibody for Western Blotting

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
779
423
358
181
40
25
19
8
8
7
3
3
2
1
1
1
Host
Mouse
471
306
39
2
2
1
Clonality
Monoclonal
Conjugate
This CDKN1B antibody is un-conjugated
40
35
35
34
33
29
9
7
7
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
629
338
240
231
199
196
102
93
84
53
37
17
14
5
5
3
1
1
1
Supplier
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Catalog No. ABIN3023777
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN3026470 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SX53G8 0
4 ABIN293608 ELISA WB Goat AA 185-198 Log in to see Polyclonal 0
4 ABIN322253 WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN3027037 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SPM348 0
1 ABIN6174384 FACS IF IHC (f) WB CF®555 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174388 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174336 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174342 FACS IF IHC (f) WB CF®543 Mouse IgG1, kappa Log in to see DCS-72-F6 0
1 ABIN6174412 FACS IF IHC (f) WB CF®405S Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174422 FACS IF IHC (f) WB CF®555 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174420 FACS IF IHC (f) WB CF®647 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174424 FACS IF IHC (f) WB CF®660R Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174430 FACS IF IHC (f) WB CF®680 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174378 FACS IF IHC (f) WB CF®640R Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174382 FACS IF IHC (f) WB CF®647 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174390 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174396 FACS IF IHC (f) WB CF®488A Mouse IgG1, kappa Log in to see KIP1-769 0
1 ABIN6174418 FACS IF IHC (f) WB CF®543 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174426 FACS IF IHC (f) WB CF®568 Mouse IgG1, kappa Log in to see SX53G8 0
1 ABIN6174428 FACS IF IHC (f) WB CF®770 Mouse IgG1, kappa Log in to see SX53G8 0

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(779), (423), (358), (181), (40), (25), (19), (8), (8), (7), (3), (3), (2), (1), (1), (1)
Host Mouse
(471), (306), (39), (2), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1B antibody is un-conjugated
(40), (35), (35), (34), (33), (29), (9), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(629), (338), (240), (231), (199), (196), (102), (93), (84), (53), (37), (17), (14), (5), (5), (3), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Characteristics This mAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
Clone KIP1-769
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name p27Kip1 (CDKN1B Antibody Abstract)
Background This mAb recognizes a 27  kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Optimal dilution of the p27Kip1 antibody to be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody (ABIN3023777) Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Gr...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody (ABIN3023777) Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 antibody...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody (ABIN3023777) Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody (ABIN3023777) Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody...
Immunohistochemistry (IHC) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody (ABIN3023777) Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (KIP1/769)