anti-Mouse (Murine) CDKN1B antibody for Western Blotting

Recommended CDKN1B Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
  • CDKN4
  • KIP1
  • MEN1B
  • MEN4
  • P27KIP1
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • CDKN1B
  • Kip1
  • p27
  • Cdki1b
  • AA408329
  • AI843786
  • p27Kip1
  • cdkn1bl
  • cyclin dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Bb
  • cyclin-dependent kinase inhibitor 1B
  • cyclin-dependent kinase inhibitor 1Ba
  • CDKN1B
  • cdkn1bb
  • Cdkn1b
  • cdkn1ba
Epitope
Center
78
58
38
29
26
25
20
19
19
19
18
17
16
14
13
10
7
7
6
6
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
662
313
254
115
24
19
8
8
7
3
3
2
1
1
Host
Rabbit
465
195
38
2
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
35
30
30
30
29
29
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
534
306
216
145
142
101
92
84
59
17
15
13
5
4
3
2
1
1
1
Options
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Available images

Catalog No. ABIN2854753
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.215494 ABIN672621 FACS IF (p) IHC (p) WB Rabbit IgG AA 150-198 Log in to see Polyclonal 3
19.715494 ABIN6271270 ELISA ICC IF IHC WB Rabbit IgG pSer178 Log in to see Polyclonal 0
18.215494 ABIN967391 BI IP WB Mouse IgG1 Log in to see G173-524 6
16 ABIN3023777 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see KIP1-769 0
13.715493 ABIN6256493 ELISA ICC IF IHC WB Rabbit IgG pThr187 Log in to see Polyclonal 0
13 ABIN3027034 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see KIP27-1 0
13 ABIN3023779 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see KIP1-769 0
12.215493 ABIN6263944 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 2
12.215493 ABIN4898980 CyTOF FACS WB Goat IgG AA 1-198 Log in to see Polyclonal 1
12.035782 ABIN4902058 ELISA WB Mouse IgG1 Log in to see 5B4-E6-E11-A8 0
12.035782 ABIN350689 IHC WB Rabbit C-Term, AA 100-170 Log in to see Polyclonal 5
12.035782 ABIN350690 IHC WB Rabbit IgG C-Term, AA 100-170 Log in to see Polyclonal 5
10.715493 ABIN2705081 IC IF IHC WB Rabbit C-Term, pSer187 Log in to see Polyclonal 0
10.715493 ABIN6254921 ELISA ICC IF WB Rabbit IgG pSer10 Log in to see Polyclonal 0
10 ABIN3026470 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see SX53G8 0
10 ABIN5575096 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see DCS-72-F6 0
10 ABIN5575097 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see DCS-72-F6 0
10 ABIN3027036 FACS IF IHC (p) WB Mouse IgG1, kappa Log in to see KIP27-1 0
9.215493 ABIN4898981 CyTOF FACS WB Mouse IgG2b AA 1-198 Log in to see 225501 2
9.215493 ABIN1881620 WB Rabbit AA 1-30, N-Term, Ser12 Log in to see Polyclonal 4

General

Antigen Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) Antibodies
Epitope Center
(78), (58), (38), (29), (26), (25), (20), (19), (19), (19), (18), (17), (16), (14), (13), (10), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(662), (313), (254), (115), (24), (19), (8), (8), (7), (3), (3), (2), (1), (1)
Host Rabbit
(465), (195), (38), (2), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1B antibody is un-conjugated
(35), (30), (30), (30), (29), (29), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(534), (306), (216), (145), (142), (101), (92), (84), (59), (17), (15), (13), (5), (4), (3), (2), (1), (1), (1)
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Product Details anti-CDKN1B Antibody

Target Details CDKN1B Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to p27 Kip1 (cyclin-dependent kinase inhibitor 1B (p27, Kip1))
p27 Kip1 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human p27 Kip1. The exact sequence is proprietary.
Isotype IgG
Plasmids, Primers & others

Target Details CDKN1B

Product Details anti-CDKN1B Antibody Application Details Handling Images back to top
Antigen
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 22 kDa
Gene ID 1027
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1B Antibody Target Details CDKN1B Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: MCF-7 , NIH-3T3 , CCRF-SB , MFB (mouse fibroblast) , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Restrictions For Research Use only

Handling

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Images back to top
Format Liquid
Concentration 0.96 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CDKN1B Antibody Target Details CDKN1B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Whole c...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: CCRF-SB 12% SDS PAGE antibody diluted...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image Sample (50 ug of whole cell lysate) A: MFB (mouse fibroblast) 12% SDS PAGE a...
Immunoprecipitation (IP) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) IP Image Immunoprecipitation of p27 Kip1 protein from MCF-7 whole cell extracts using...
Immunofluorescence (IF) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) ICC/IF Image p27 Kip1 antibody detects p27 Kip1 protein at cytoplasm and nucleus by i...
Western Blotting (WB) image for anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (Center) antibody (ABIN2854753) WB Image p27 Kip1 antibody detects p27 Kip1 protein by western blot analysis. Various...