anti-Human SMAD3 antibody for Flow Cytometry

Recommended SMAD3 Antibody (supplied by: Log in to see )

Antigen
SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Epitope
AA 2-230
72
61
52
49
38
36
22
22
19
18
18
16
12
11
11
11
10
9
8
7
7
7
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
616
334
318
57
48
42
39
32
23
19
18
4
3
3
2
2
1
1
1
1
1
Host
Rat
560
64
14
Clonality
Monoclonal
Conjugate
This SMAD3 antibody is un-conjugated
24
22
20
12
12
11
8
8
7
7
7
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
488
272
263
135
112
67
56
35
31
19
18
9
7
2
2
1
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Catalog No. ABIN4899431
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.729485 ABIN653953 FACS IF IHC (p) WB Rabbit Ig Fraction AA 186-215, Ser208 Log in to see Polyclonal 2
13.729485 ABIN5947588 FACS ICC IF IHC IHC (p) WB Rabbit IgG AA 1-200 Log in to see SY25-01 0
1 ABIN4354683 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 2
1 ABIN214557 ELISA FACS IF IHC IHC (p) WB Rabbit Ser425, pSer423 Log in to see Polyclonal 0
1 ABIN5556330 FACS IF IHC (p) WB Rabbit Ig Fraction Ser208 Log in to see Polyclonal 0
1 ABIN5531927 FACS IF IHC (p) WB Rabbit Ig Fraction AA 186-215 Log in to see Polyclonal 0
1 ABIN3032621 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 186-215 Log in to see Polyclonal 0
1 ABIN4354682 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN222350 FACS IF IP ELISA WB Rabbit IgG N-Term Log in to see 0
1 ABIN4354691 FACS Alexa Fluor 750 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354692 FACS Alexa Fluor 594 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354693 FACS Alexa Fluor 350 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354689 FACS Alexa Fluor 700 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354688 FACS Alexa Fluor 488 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354690 FACS Alexa Fluor 647 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN4354694 FACS Alexa Fluor 405 Rat IgG1 AA 2-230 Log in to see 378611 0
1 ABIN1957337 ELISA FACS ICC IF IHC WB HRP Rabbit IgG AA 186-215 Log in to see Polyclonal 0
1 ABIN1956113 ELISA FACS ICC IF IHC WB FITC Rabbit IgG AA 186-215 Log in to see Polyclonal 0
1 ABIN1952796 ELISA FACS ICC IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG AA 186-215 Log in to see Polyclonal 0
1 ABIN1954963 ELISA FACS ICC IF IHC WB Biotin Rabbit IgG AA 186-215 Log in to see Polyclonal 0

General

Antigen SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
Epitope AA 2-230
(72), (61), (52), (49), (38), (36), (22), (22), (19), (18), (18), (16), (12), (11), (11), (11), (10), (9), (8), (7), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(616), (334), (318), (57), (48), (42), (39), (32), (23), (19), (18), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rat
(560), (64), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SMAD3 antibody is un-conjugated
(24), (22), (20), (12), (12), (11), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
(488), (272), (263), (135), (112), (67), (56), (35), (31), (19), (18), (9), (7), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-SMAD3 Antibody

Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN4899431) Images
Specificity Detects human Smad3 in direct ELISAs.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human Smad3, Ser2-Ala230, Accession # P84022
Clone 378611
Isotype IgG1
Plasmids, Primers & others

Target Details SMAD3

Product Details anti-SMAD3 Antibody Application Details Handling References for anti-SMAD3 antibody (ABIN4899431) Images back to top
Antigen
Alternative Name Smad3 (SMAD3 Antibody Abstract)
Background Alternative Names: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene ID 4088
UniProt P84022
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy

Application Details

Product Details anti-SMAD3 Antibody Target Details SMAD3 Handling References for anti-SMAD3 antibody (ABIN4899431) Images back to top
Application Notes Immunohistochemistry 8-25 μg/mL
Intracellular Staining by Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details References for anti-SMAD3 antibody (ABIN4899431) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-SMAD3 antibody (ABIN4899431)

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling Images back to top
Product cited in:

Yeh, Kuo, Chan, Chang, Qi, Tsai, Nattel, Chen: "Transforming growth factor-? and oxidative stress mediate tachycardia-induced cellular remodelling in cultured atrial-derived myocytes." in: Cardiovascular research, Vol. 91, Issue 1, pp. 62-70, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN4899431) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 2-230) antibody (ABIN4899431) Detection of Smad3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate can...
Immunocytochemistry (ICC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 2-230) antibody (ABIN4899431) Smad3 in MDA-MB-231 Human Cell Line. Smad3 was detected in immersion fixed MDA-MB-231...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 2-230) antibody (ABIN4899431) Smad3 in Human Pancreatic Cancer Tissue. Smad3 was detected in immersion fixed paraff...