anti-Human SMAD3 antibody for Immunohistochemistry

Recommended SMAD3 Antibody (supplied by: Log in to see )

Antigen
SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Epitope
AA 417-425, pSer425, pSer423
71
60
52
49
38
36
22
22
19
18
18
16
12
11
11
10
10
9
8
8
7
7
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
616
334
318
57
48
42
39
32
23
19
18
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
559
64
15
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
24
22
20
12
12
11
8
8
7
7
7
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
487
272
262
135
112
67
57
36
31
19
18
9
7
2
2
1
1
Options
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Catalog No. ABIN129675
$ 389.00
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.673489 ABIN2792537 IHC WB Rabbit N-Term Log in to see Polyclonal 1
8.673489 ABIN2707070 IHC WB Rabbit Center Log in to see Polyclonal 0
8.673489 ABIN2705246 IHC WB Rabbit C-Term, pSer425 Log in to see Polyclonal 0
8.673489 ABIN2707071 IC IF IHC WB Rabbit Center Log in to see Polyclonal 0
8.673489 ABIN2707068 IHC WB Rabbit pSer179 Log in to see Polyclonal 0
8.673489 ABIN4899431 CyTOF FACS ICC IHC Rat IgG1 AA 2-230 Log in to see 378611 1
8.673489 ABIN4354683 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 2
8.673489 ABIN1531631 IHC ELISA WB Rabbit IgG AA 145-194, pThr179 Log in to see Polyclonal 1
8.673489 ABIN1532654 IHC ELISA WB Rabbit IgG AA 170-219 Log in to see Polyclonal 1
8.673489 ABIN1531633 IHC ELISA WB Rabbit IgG AA 174-223, pSer208 Log in to see Polyclonal 0
8.673489 ABIN1531200 IHC ELISA WB Rabbit IgG AA 376-425, pSer425 Log in to see Polyclonal 0
8.673489 ABIN1532216 IHC ELISA WB Rabbit IgG AA 376-425 Log in to see Polyclonal 0
8.673489 ABIN129676 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 1
8.673489 ABIN2852667 IHC WB Mouse Log in to see 0
8.673489 ABIN2470312 IF IHC ELISA WB Rabbit AA 417-425 Log in to see Polyclonal 0
8.673489 ABIN4354684 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
8.673489 ABIN2470142 IHC WB Rabbit AA 100-150 Log in to see Polyclonal 0
8.673489 ABIN3188041 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.673489 ABIN3181562 IHC WB Rabbit IgG Log in to see Polyclonal 0
8.673489 ABIN3032620 IF IHC ELISA WB Rabbit Ig Fraction AA 127-156 Log in to see Polyclonal 0

General

Antigen SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
Epitope AA 417-425, pSer425, pSer423
(71), (60), (52), (49), (38), (36), (22), (22), (19), (18), (18), (16), (12), (11), (11), (10), (10), (9), (8), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(616), (334), (318), (57), (48), (42), (39), (32), (23), (19), (18), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(559), (64), (15)
Clonality
Polyclonal
Conjugate This SMAD3 antibody is un-conjugated
(24), (22), (20), (12), (12), (11), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(487), (272), (262), (135), (112), (67), (57), (36), (31), (19), (18), (9), (7), (2), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-SMAD3 Antibody

Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN129675) Images
Cross-Reactivity Xenopus laevis, Xenopus tropicalis, Zebrafish (Brachydanio rerio), Rat (Rattus), Mouse (Murine), Pig (Porcine), Sheep (Ovine), Chicken
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 417-425 of human SMAD3 protein.
Isotype IgG
Plasmids, Primers & others

Target Details SMAD3

Product Details anti-SMAD3 Antibody Application Details Handling References for anti-SMAD3 antibody (ABIN129675) Images back to top
Antigen
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase.   These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the BMP pathway.  Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated response.
Synonyms: hMAD 3 antibody, hSMAD3 antibody, MADH3 antibody, MGC60396 antibody, Mothers against decapentaplegic homolog 3 antibody, Mothers against DPP homolog 3 antibody
Gene ID 4088, 5174513
UniProt P84022
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy

Application Details

Product Details anti-SMAD3 Antibody Target Details SMAD3 Handling References for anti-SMAD3 antibody (ABIN129675) Images back to top
Application Notes This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to phosphorylated Smad3 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.  Less than 0.2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for dual phosphorylated pS423 and pS425 of Smad3. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
Restrictions For Research Use only

Handling

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details References for anti-SMAD3 antibody (ABIN129675) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-SMAD3 antibody (ABIN129675)

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling Images back to top
Product cited in:

Beckham, Tuttle, Tyler: "Reovirus activates transforming growth factor beta and bone morphogenetic protein signaling pathways in the central nervous system that contribute to neuronal survival following infection." in: Journal of virology, Vol. 83, Issue 10, pp. 5035-45, 2009

Coffman, Coluccio, Planchart, Robertson: "Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2." in: Developmental biology, Vol. 330, Issue 1, pp. 123-30, 2009

Yamashita, Fatyol, Jin, Wang, Liu, Zhang: "TRAF6 mediates Smad-independent activation of JNK and p38 by TGF-beta." in: Molecular cell, Vol. 31, Issue 6, pp. 918-24, 2008

Images

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN129675) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 µg/ml to detect sig...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) 1.17 mg/ml (by UV absorbance at 280 nm)
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (Image 3)
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (Image 4)
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) Western Blot of Rabbit anti-SMAD3 pS423 pS425 antibody. Marker: Opal Pre-stained ladd...
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) Western blot using affinity purified anti-Smad3 pS423 pS425 antibody shows detection...
 image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody (ABIN129675) affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 ug/ml to detect sig...