anti-Mouse (Murine) SMAD3 antibody for Flow Cytometry

Recommended SMAD3 Antibody (supplied by: Log in to see )

Antigen
SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
  • madh3
  • madh3a
  • smad3
  • wu:fa99e03
  • HSPC193
  • HsT17436
  • JV15-2
  • LDS1C
  • LDS3
  • MADH3
  • XSmad3
  • XenMLP
  • Xmad3
  • madh3-A
  • Madh3
  • AU022421
  • Smad 3
  • mad3
  • SMAD family member 3
  • SMAD family member 3a
  • SMAD family member 3 L homeolog
  • SMAD3
  • smad3a
  • smad3.L
  • Smad3
Epitope
AA 186-215, Ser208
72
61
52
49
38
36
22
22
19
18
17
16
11
11
11
11
10
9
8
8
7
7
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
616
333
318
57
48
42
39
32
23
19
18
4
3
3
2
2
1
1
1
1
1
Host
Rabbit
559
64
15
Clonality
Polyclonal
Conjugate
This SMAD3 antibody is un-conjugated
24
22
20
12
12
11
8
8
7
7
7
5
5
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
487
273
263
134
111
67
56
36
31
19
18
9
7
2
2
1
1
Options
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Catalog No. ABIN653953
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.729507 ABIN5947588 FACS ICC IF IHC IHC (p) WB Rabbit IgG AA 1-200 Log in to see SY25-01 0
1 ABIN4354683 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 2
1 ABIN214557 ELISA FACS IF IHC IHC (p) WB Rabbit Ser425, pSer423 Log in to see Polyclonal 0
1 ABIN5531927 FACS IF IHC (p) WB Rabbit Ig Fraction AA 186-215 Log in to see Polyclonal 0
1 ABIN3032621 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 186-215 Log in to see Polyclonal 0
1 ABIN4354682 ELISA FACS IHC IHC (p) WB Rabbit pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN222350 FACS IF IP ELISA WB Rabbit IgG N-Term Log in to see 0
1 ABIN5663003 ELISA FACS IHC IHC (p) WB Alexa Fluor 405 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663004 ELISA FACS IHC IHC (p) WB Alexa Fluor 488 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663005 ELISA FACS IHC IHC (p) WB Alexa Fluor 647 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663007 FACS APC Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663006 ELISA FACS IHC IHC (p) WB Alexa Fluor 700 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663009 ELISA FACS IHC IHC (p) WB DyLight 350 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663010 ELISA FACS IHC IHC (p) WB DyLight 405 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663011 ELISA FACS IHC IHC (p) WB DyLight 488 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663012 ELISA FACS IHC IHC (p) WB DyLight 550 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663013 ELISA FACS IHC IHC (p) WB DyLight 650 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663014 ELISA FACS IHC IHC (p) WB DyLight 680 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663015 ELISA FACS IHC IHC (p) WB DyLight 755 Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0
1 ABIN5663016 ELISA FACS IHC IHC (p) FITC Rabbit IgG pSer423, pSer425 Log in to see Polyclonal 0

General

Antigen SMAD, Mothers Against DPP Homolog 3 (SMAD3) Antibodies
Epitope AA 186-215, Ser208
(72), (61), (52), (49), (38), (36), (22), (22), (19), (18), (17), (16), (11), (11), (11), (11), (10), (9), (8), (8), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(616), (333), (318), (57), (48), (42), (39), (32), (23), (19), (18), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(559), (64), (15)
Clonality (Clone)
Polyclonal   ( )
Conjugate This SMAD3 antibody is un-conjugated
(24), (22), (20), (12), (12), (11), (8), (8), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(487), (273), (263), (134), (111), (67), (56), (36), (31), (19), (18), (9), (7), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-SMAD3 Antibody

Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN653953) Images
Predicted Reactivity Chicken,Pig (Porcine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 AA from human SMAD3.
Clone RB24758
Isotype Ig Fraction
Plasmids, Primers & others

Target Details SMAD3

Product Details anti-SMAD3 Antibody Application Details Handling References for anti-SMAD3 antibody (ABIN653953) Images back to top
Antigen
Alternative Name SMAD3 (SMAD3 Antibody Abstract)
Background Synonyms: JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, MAD homolog 3, Mad3, hMAD-3,MADH3, SMAD3
Molecular Weight 48081 DA
Gene ID 4088
UniProt P84022
Pathways Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy

Application Details

Product Details anti-SMAD3 Antibody Target Details SMAD3 Handling References for anti-SMAD3 antibody (ABIN653953) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50, IF = 1:10-50, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details References for anti-SMAD3 antibody (ABIN653953) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-SMAD3 antibody (ABIN653953)

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling Images back to top
Product cited in:

Zhou, Liu, Pan, Du, Li, Ma, Yao, Li, Liu: "The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-? type 1 receptor." in: Molecular and cellular endocrinology, Vol. 409, pp. 103-12, 2015

Lessard, Rivas, Alves-Wagner, Hirshman, Gallagher, Constantin-Teodosiu, Atkins, Greenhaff, Qi, Gustafsson, Fielding, Timmons, Britton, Koch, Goodyear: "Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks." in: Diabetes, Vol. 62, Issue 8, pp. 2717-27, 2013

Images

Product Details anti-SMAD3 Antibody Target Details SMAD3 Application Details Handling References for anti-SMAD3 antibody (ABIN653953) back to top
Supplier Images
Western Blotting (WB) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215), (Ser208) antibody (ABIN653953) Western blot analysis of hSMAD3-S208 (ABIN653953) in MDA-MB231 cell line lysates (35 ...
Immunohistochemistry (IHC) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215), (Ser208) antibody (ABIN653953) SMAD3-S208 Antibody (ABIN653953) IHC analysis in formalin fixed and paraffin embedded...
Flow Cytometry (FACS) image for anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 186-215), (Ser208) antibody (ABIN653953) SMAD3-S208 Antibody (ABIN653953) flow cytometric analysis of MDA-MB231 cells (right h...