anti-Human RAG2 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended RAG2 Antibody (supplied by: Log in to see )

Antigen
Recombination Activating Gene 2 (RAG2) Antibodies
  • RAG-2
  • Rag-2
  • rag-2
  • zgc:136743
  • recombination activating gene 2
  • RAG2
  • Rag2
  • rag2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
61
32
26
2
2
1
1
1
1
Host
Rabbit
43
18
Clonality
Polyclonal
Conjugate
This RAG2 antibody is conjugated to Cy7
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p))
48
20
15
13
12
10
8
4
1
1
1
1
1
1
Options
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Catalog No. ABIN912888
$ 405.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN912898 IF (p) Cy3 Rabbit IgG Log in to see Polyclonal
1 ABIN912901 IF (p) Alexa Fluor 647 Rabbit IgG Log in to see Polyclonal
1 ABIN912903 IF (p) Alexa Fluor 555 Rabbit IgG Log in to see Polyclonal
1 ABIN912895 IF (p) Cy5.5 Rabbit IgG Log in to see Polyclonal
1 ABIN912892 IF (p) Alexa Fluor 350 Rabbit IgG Log in to see Polyclonal
1 ABIN912897 IF (p) Cy5 Rabbit IgG Log in to see Polyclonal
1 ABIN912900 IF (p) FITC Rabbit IgG Log in to see Polyclonal
1 ABIN912899 IF (p) Alexa Fluor 488 Rabbit IgG Log in to see Polyclonal
1 ABIN2805783 IF (p) Alexa Fluor 594 Rabbit IgG Log in to see Polyclonal
1 ABIN873003 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN5009547 IF (p) Alexa Fluor 750 Rabbit IgG Log in to see Polyclonal
1 ABIN5009546 IF (p) Alexa Fluor 680 Rabbit IgG Log in to see Polyclonal

General

Antigen Recombination Activating Gene 2 (RAG2) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(61), (32), (26), (2), (2), (1), (1), (1), (1)
Host Rabbit
(43), (18)
Clonality
Polyclonal
Conjugate This RAG2 antibody is conjugated to Cy7
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p))
(48), (20), (15), (13), (12), (10), (8), (4), (1), (1), (1), (1), (1), (1)
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Product Details anti-RAG2 Antibody

Target Details RAG2 Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human RAG2
Isotype IgG

Target Details RAG2

Product Details anti-RAG2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Rag2 (RAG2 Antibody Abstract)
Background

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

Synonyms: RAG 2, RAG-2, RAG2, RAG2_HUMAN, Recombination activating gene 2, VDJ recombination activating protein 2, VDJ recombination-activating protein 2.

Gene ID 5897
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways Chromatin Binding, Production of Molecular Mediator of Immune Response

Application Details

Product Details anti-RAG2 Antibody Target Details RAG2 Handling Images back to top
Application Notes IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-RAG2 Antibody Target Details RAG2 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C
Expiry Date 12 months